| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Hepatic leukemia factor |
| Entrez GeneID | 3131 |
| WB Predicted band size | Calculated MW: 33 kDa; Observed MW: 33 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human HLF |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于HLF(Hepatic Leukemia Factor)抗体的3篇参考文献及其摘要信息：
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1. **文献名称**: "The role of HLF in hepatic and hematopoietic malignancies"
**作者**: Inaba T, et al.
**摘要**: 该研究探讨了HLF在急性淋巴细胞白血病(ALL)和肝细胞癌中的异常表达机制，通过HLF抗体的免疫沉淀实验，揭示了HLF融合蛋白与DNA结合的异常模式及其致癌作用。
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2. **文献名称**: "PAR bZIP transcription factors interact with HLF during liver regeneration"
**作者**: Hansen J, et al.
**摘要**: 研究利用HLF抗体进行染色质免疫共沉淀(ChIP)，发现HLF与PAR bZIP家族蛋白在小鼠肝损伤修复中协同调控细胞周期相关基因，提示HLF在肝脏再生中的关键功能。
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3. **文献名称**: "Antibody-based detection of HLF isoforms in pediatric leukemia"
**作者**: Smith R, et al.
**摘要**: 通过开发高特异性HLF抗体，该文献对比了不同HLF剪接变体在儿童白血病样本中的表达差异，证明特定HLF异构体可作为预后标志物，并优化了临床检测流程。
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**备注**：以上文献信息为示例，实际引用时需核实具体来源及细节。如需进一步精准文献，建议通过PubMed或学术数据库检索关键词“HLF antibody”或“Hepatic Leukemia Factor”。
**Background of HLF Antibody**
The **hepatic leukemia factor (HLF)** antibody is a tool used to detect and study HLF, a transcription factor belonging to the proline- and acidic amino acid-rich (PAR) bZIP family. HLF contains a basic leucine zipper (bZIP) domain critical for DNA binding and dimerization, and an N-terminal activation domain regulating gene expression. It plays roles in circadian rhythm regulation, cellular stress responses, and tissue-specific functions, particularly in the liver, kidneys, and intestines.
HLF gained attention due to its involvement in **acute lymphoblastic leukemia (ALL)**. Chromosomal translocations, such as t(17;19), fuse *HLF* with *TCF3* (E2A), generating the oncogenic TCF3-HLF fusion protein. This aberrant protein drives leukemogenesis by disrupting normal transcriptional programs, promoting cell survival and proliferation. HLF antibodies are thus pivotal in diagnosing fusion-positive ALL and studying its molecular mechanisms.
Beyond oncology, HLF antibodies aid research in **tissue regeneration** and **metabolic regulation**, as HLF influences hepatocyte function and stress adaptation. Recent studies also explore its role in neurodegenerative diseases and solid tumors. By enabling HLF detection in tissues or cell lines, these antibodies support efforts to unravel its dual roles in health and disease, offering potential therapeutic insights.
In summary, HLF antibodies are essential for understanding HLF's biological functions, its pathological contributions to leukemia, and broader implications in cellular stress and homeostasis.
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