| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FIH 1; FIH1; HIF1AN |
| Entrez GeneID | 55662 |
| WB Predicted band size | Calculated MW: 40 kDa; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human HIF1AN/FIH-1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于HIF1N抗体的3篇示例参考文献(内容为模拟概括，非真实文献)：
---
1. **文献名称**：*HIF1AN suppresses ovarian cancer progression via hydroxylation-dependent HIF-1α degradation*
**作者**：Zhang L, et al.
**摘要**：本研究通过Western blot和免疫组化技术，利用HIF1AN抗体证实其在卵巢癌组织中表达下调，并通过羟基化修饰促进HIF-1α蛋白降解，抑制肿瘤血管生成和转移，提示其作为预后标志物的潜力。
2. **文献名称**：*HIF1AN-mediated regulation of hypoxic response in glioblastoma stem cells*
**作者**：Wang Y, et al.
**摘要**：作者使用HIF1AN抗体进行免疫共沉淀(Co-IP)和ChIP-seq分析，发现HIF1AN通过抑制胶质母细胞瘤干细胞中HIF-1α的转录活性，调控肿瘤干性维持和化疗耐药性。
3. **文献名称**：*Structural and functional characterization of HIF1AN as an oxygen-sensitive epigenetic regulator*
**作者**：Smith J, et al.
**摘要**：该研究解析了HIF1AN蛋白结构，并通过HIF1AN抗体验证其与组蛋白乙酰转移酶p300的相互作用，表明其在低氧条件下通过表观遗传修饰调控靶基因表达。
---
**备注**：以上文献为示例，实际研究中建议通过PubMed或Web of Science检索真实文献(关键词：HIF1AN antibody, hydroxylase, hypoxia)。
The HIF1AN (Hypoxia-Inducible Factor 1 Alpha Inhibitor) antibody is a key tool for studying the regulation of cellular responses to hypoxia. HIF1AN, also known as Factor Inhibiting HIF-1 (FIH-1), is an oxygen-sensitive enzyme that post-translationally modifies hypoxia-inducible factor 1α (HIF-1α). Under normoxic conditions, HIF1AN hydroxylates HIF-1α at asparagine residues, blocking its interaction with transcriptional coactivators like p300/CBP, thereby suppressing HIF-1-mediated gene expression. This activity complements the oxygen-dependent degradation of HIF-1α mediated by prolyl hydroxylases (PHDs). HIF1AN also hydroxylates other substrates, including proteins involved in the Notch signaling pathway, indicating broader regulatory roles in metabolism, angiogenesis, and tumor progression.
Antibodies targeting HIF1AN are widely used in research to investigate its expression, subcellular localization, and interactions in various physiological and pathological contexts, such as cancer, ischemic diseases, and metabolic disorders. They enable techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation. Validated HIF1AN antibodies typically show specificity for human, mouse, or rat isoforms, with applications in both normal and hypoxia-mimicking conditions. Understanding HIF1AN dynamics aids in exploring therapeutic strategies targeting HIF-1 signaling pathways, which are implicated in tumor growth and adaptation to low-oxygen microenvironments.
×
