| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | YT; ACEE; ARACHE; N-ACHE |
| Entrez GeneID | 43 |
| clone | 3D5E8 |
| WB Predicted band size | 67.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Monkey, Rat |
| Immunogen | Purified recombinant fragment of human ACHE expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CEP55抗体的3篇参考文献示例(注：文献信息为模拟生成，实际引用请核实数据库)：
1. **文献名称**：*CEP55 regulates cytokinesis and genome stability by recruiting ESCRT-III to the midbody*
**作者**：Zhao, W.M., et al.
**摘要**：本研究利用CEP55特异性抗体进行免疫荧光和Western blot分析，揭示了CEP55在胞质分裂中招募ESCRT-III复合体的关键作用，并证明其缺失会导致基因组不稳定和多核细胞形成。
2. **文献名称**：*Overexpression of CEP55 predicts poor prognosis in hepatocellular carcinoma*
**作者**：Jiang, Y., et al.
**摘要**：通过免疫组化(使用CEP55抗体)分析肝癌组织样本，发现CEP55在肿瘤组织中显著过表达，其高表达与患者生存期缩短相关，提示CEP55可作为肝癌预后生物标志物。
3. **文献名称**：*CEP55 promotes tumorigenesis in triple-negative breast cancer via PI3K/AKT pathway activation*
**作者**：Chen, L., et al.
**摘要**：研究通过shRNA敲低和CEP55抗体介导的蛋白检测，证实CEP55通过激活PI3K/AKT信号通路促进三阴性乳腺癌增殖和转移，为靶向治疗提供潜在靶点。
**建议**：实际文献可通过PubMed或Google Scholar搜索关键词“CEP55 antibody”、“CEP55 function”结合近年筛选。部分经典研究可能发表于*Journal of Cell Biology*、*Oncogene*等期刊。
CEP55 (Centrosomal Protein 55) is a key regulator of cell division, primarily involved in the final stages of cytokinesis. Encoded by the CEP55 gene, this protein localizes to the centrosome, midbody, and midbody ring during mitosis. Structurally, it contains a coiled-coil domain and a conserved C-terminal "CEP55" domain, enabling interactions with proteins critical for abscission, the physical separation of daughter cells. CEP55 recruits components of the endosomal sorting complex required for transport (ESCRT) machinery, such as TSG101 and ALIX, to facilitate membrane scission. Dysregulation of CEP55 disrupts cytokinesis, leading to genomic instability, aneuploidy, and multinucleated cells—hallmarks of cancer.
CEP55 overexpression is observed in various cancers (e.g., breast, liver, lung) and correlates with poor prognosis, tumor aggressiveness, and chemoresistance. Its role in oncogenesis extends beyond cytokinesis; it also modulates signaling pathways like PI3K/AKT and Wnt/β-catenin. Conversely, CEP55 mutations or deficiencies are linked to developmental disorders, including Meckel-Gruber syndrome.
CEP55-specific antibodies are vital tools for studying mitotic mechanisms, cancer biology, and disease diagnostics. They enable detection of CEP55 expression and localization via techniques like immunofluorescence, Western blotting, and immunohistochemistry. Research using these antibodies has advanced understanding of cell cycle regulation, tumorigenesis, and potential therapeutic targeting of CEP55 in precision oncology.
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