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Rabbit Monoclonal AMSH Antibody

  • 中文名： AMSH抗体
  • 别    名： AMSH; MICCAP
货号: IPDX22253
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesAMSH; MICCAP
Entrez GeneID10617
WB Predicted band sizeCalculated MW: 48 kDa; Observed MW: 48 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthetic peptide of human AMSH
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于AMSH抗体的3篇虚构参考文献示例(仅供格式参考)：

1. **文献名称**：*AMSH regulates endosomal receptor trafficking via its role in deubiquitination*

**作者**：K. Tanaka, et al.

**摘要**：本研究揭示了AMSH蛋白通过其去泛素化酶活性调控EGFR等受体的内体分选过程，并开发了特异性识别AMSH的抗体用于免疫印迹分析，验证了其在细胞迁移中的功能。

2. **文献名称**：*Structural basis for AMSH-mediated STAM interaction in cytokine signaling*

**作者**：M. Sato, et al.

**摘要**：通过晶体学解析AMSH与STAM的SH3结构域复合物结构，利用单克隆抗体定位关键结合区域，证实该互作对JAK-STAT信号通路的负向调控作用。

3. **文献名称**：*AMSH antibody-based detection reveals elevated expression in breast cancer tissues*

**作者**：L. Chen, et al.

**摘要**：通过组织芯片免疫组化分析发现AMSH在乳腺癌样本中异常高表达，其多克隆抗体可特异性识别病理切片中的AMSH蛋白，提示其作为潜在生物标志物的可能性。

(注：以上文献为模拟示例，实际文献需通过PubMed/Google Scholar等平台检索)

背景信息

AMSH (Associated Molecule with the SH3 domain of STAM) is a deubiquitinating enzyme involved in regulating endosomal sorting and receptor trafficking. It interacts with components of the ESCRT (Endosomal Sorting Complex Required for Transport) machinery, playing a critical role in controlling lysosomal degradation of ubiquitinated membrane proteins, such as activated growth factor receptors. AMSH preferentially cleaves K63-linked ubiquitin chains, a modification often associated with endocytic sorting signals, thereby influencing processes like EGFR (epidermal growth factor receptor) downregulation. Structurally, AMSH contains a JAMM (JAB1/MPN/Mov34 metalloenzyme) domain responsible for its catalytic activity and an N-terminal microtubule-interacting domain. Dysregulation of AMSH has been linked to pathologies, including cancer and neurodegenerative disorders, due to its impact on cell signaling and protein homeostasis. Antibodies targeting AMSH are widely used in research to study endocytosis, ubiquitin-dependent sorting mechanisms, and cellular stress responses. They enable detection of AMSH expression, localization, and interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Specific clones are validated for distinguishing between isoforms or post-translationally modified forms of AMSH, aiding in mechanistic studies of its role in membrane trafficking and disease.

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