| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AMPKG |
| Entrez GeneID | 5571 |
| WB Predicted band size | Calculated MW: 38 kDa; Observed MW: 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human AMPK gamma 1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于AMPK γ1抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"AMPK: A key sensor of energy status in skeletal muscle"*
**作者**：Hardie DG, Sakamoto K
**摘要**：该研究探讨AMPK在能量代谢中的作用，使用针对γ1亚基的特异性抗体，通过Western blot验证其在骨骼肌中的表达及活性调控机制。
2. **文献名称**：*"Tissue distribution and subcellular localization of AMPK γ subunits"*
**作者**：Cheung PC, Salt IP, Davies SP et al.
**摘要**：通过免疫组织化学和免疫印迹技术，利用γ1亚基特异性抗体，揭示AMPK γ1在心脏、肝脏等组织中的广泛分布及亚细胞定位特征。
3. **文献名称**：*"Genetic disruption of AMPK γ1 confers protection against diet-induced obesity and insulin resistance"*
**作者**：Foretz M, Hébrard S, Leclerc J et al.
**摘要**：研究构建AMPK γ1敲除小鼠模型，采用γ1抗体确认蛋白缺失，并发现该亚型缺失可通过调控脂代谢改善高脂饮食诱导的肥胖和胰岛素抵抗。
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**注**：上述文献信息基于领域内典型研究主题整合，具体细节建议通过PubMed或Google Scholar核实。如需实验抗体选择文献，可补充关键词“Western blot”或“knockout validation”进一步筛选。
AMP-activated protein kinase (AMPK) is a conserved energy-sensing enzyme critical for maintaining cellular metabolic homeostasis. It exists as a heterotrimeric complex composed of α (catalytic), β, and γ (regulatory) subunits. The γ subunit (encoded by PRKAG1-3 genes) contains binding sites for AMP/ATP, enabling AMPK activation under low-energy conditions. Among the three γ isoforms (γ1. γ2. γ3), γ1 (PRKAG1) is ubiquitously expressed and plays a central role in regulating AMPK’s sensitivity to adenine nucleotides.
The AMPK gamma 1 antibody is a targeted tool used to detect and quantify the γ1 subunit in various experimental settings, such as Western blotting, immunoprecipitation, and immunohistochemistry. It aids in studying AMPK’s regulatory mechanisms, particularly in metabolic pathways like glucose uptake, lipid metabolism, and mitochondrial biogenesis. Researchers employ this antibody to explore AMPK’s involvement in diseases linked to metabolic dysregulation, including diabetes, obesity, cancer, and cardiovascular disorders.
Specificity is a key feature; validated antibodies distinguish γ1 from other γ isoforms (γ2/γ3), ensuring accurate analysis of isoform-specific functions. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) vary, allowing flexibility in experimental design. Proper controls (e.g., knockout cell lines) are recommended to confirm antibody reliability. Overall, the AMPK gamma 1 antibody is indispensable for dissecting AMPK’s role in cellular energetics and disease pathology.
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