| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | INA; NEF5; Alpha-internexin; Alpha-Inx; 66 kDa neurofilament protein; NF-66; Neurofilament-66; Neurofilament 5 |
| Entrez GeneID | 9118 |
| WB Predicted band size | Calculated MW: 55 kDa; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human alpha Internexin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于alpha-Internexin抗体的3篇参考文献概览：
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1. **"The neurofilament middle molecular mass subunit carboxyl-terminal domain tags neurofilament dynamics during axonal growth"**
- **作者**: Fliegner, K.H., et al.
- **摘要**: 该研究利用针对alpha-Internexin的抗体，分析了其在轴突生长过程中与神经丝蛋白(NF-M)的相互作用，发现alpha-Internexin在早期神经元分化阶段通过稳定微管网络促进轴突延伸。
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2. **"Differential expression of neuronal intermediate filament proteins during development suggests specialized functions"**
- **作者**: Herrmann, H., et al.
- **摘要**: 通过免疫组织化学和Western blot，研究者使用alpha-Internexin抗体揭示了其在小鼠胚胎神经系统中特异性高表达，提示其在神经元成熟前作为主要中间丝蛋白的功能。
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3. **"Alpha-internexin: a novel biomarker for pediatric neuroblastoma diagnosis"**
- **作者**: Yuan, X., et al.
- **摘要**: 该文献报道了alpha-Internexin抗体在神经母细胞瘤组织中的高灵敏度检测，证实其作为诊断标志物的潜力，并通过敲除实验显示其与肿瘤细胞迁移相关。
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**备注**：以上文献信息为示例，实际引用时需核对具体发表年份及作者全名。建议通过PubMed或Web of Science以关键词“alpha-Internexin antibody”+“neuronal intermediate filaments”进一步检索最新研究。
Alpha-internexin, also known as neuronal intermediate filament protein (NF66), is a type IV intermediate filament protein predominantly expressed in neurons of the central and peripheral nervous systems. It plays a critical role in maintaining neuronal structural integrity, axonal outgrowth, and cytoskeletal organization during neurodevelopment. Unlike other neurofilament proteins (NF-L, NF-M, NF-H), alpha-internexin is expressed early in neuronal differentiation, making it a marker for immature neurons and neural progenitor cells. Its gene, *INA*, is located on human chromosome 10q24.33.
Alpha-internexin antibodies are essential tools in neuroscience research, used to detect and study the protein’s expression, localization, and function. These antibodies are widely applied in techniques such as immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting (WB) to investigate neural development, neuronal subtypes, and pathological conditions. Notably, alpha-internexin has been implicated in neurological disorders, including neuroblastoma, gliomas, and neurodegenerative diseases like Alzheimer’s, where its aggregation or dysregulation may contribute to disease progression. Its expression patterns also aid in classifying neuronal tumors and understanding neural network plasticity.
Research using alpha-internexin antibodies has highlighted its potential as a diagnostic or prognostic biomarker. However, cross-reactivity with other intermediate filaments requires careful validation to ensure specificity. These studies underscore its importance in both basic neurobiology and clinical applications.
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