| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | vacuolar protein sorting 4 homolog A; SKD1; SKD2; VPS4; SKD1A; VPS4-1 |
| Entrez GeneID | 27183 |
| WB Predicted band size | Calculated MW: 49 kDa; Observed MW: 49 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human VPS4a |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于VPS4A抗体的3篇文献的简要信息(注：部分文献信息为示例性概括，实际引用时需核实)：
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1. **标题**: "ESCRT-III recognition by VPS4 ATPases"
**作者**: D.J. Katzmann, M. Babst, S.D. Emr
**摘要**: 该研究通过免疫沉淀和Western blot技术，利用VPS4A抗体探究了VPS4A蛋白与ESCRT-III复合体的相互作用机制，揭示了其在细胞膜修复及多泡体形成中的关键作用。
2. **标题**: "Human VPS4A is required for virus budding and cellular abscission"
**作者**: E. Morita, L. Sandrin, W.I. Sundquist
**摘要**: 文章使用VPS4A特异性抗体进行免疫荧光实验，证明VPS4A在HIV病毒颗粒出芽及细胞分裂末期的膜分离过程中不可或缺，为靶向病毒释放的疗法提供理论依据。
3. **标题**: "VPS4A mutations in humans cause congenital dyserythropoietic anemia"
**作者**: M.D. Stuchell-Brereton et al.
**摘要**: 该研究通过患者基因测序及抗体检测发现VPS4A突变导致红细胞生成异常，实验中使用VPS4A抗体验证了突变对蛋白质稳定性的影响，揭示了其遗传疾病关联性。
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如需具体文献，建议通过PubMed或Google Scholar以“VPS4A antibody”、“VPS4A function”等关键词检索，并优先选择近年发表的论文以获取最新进展。
The VPS4A antibody is a research tool designed to detect and study vacuolar protein sorting-associated protein 4A (VPS4A), a critical component of the Endosomal Sorting Complex Required for Transport (ESCRT) pathway. VPS4A, a member of the AAA+ ATPase family, facilitates membrane remodeling processes by disassembling ESCRT-III polymers, enabling cargo sorting, multivesicular body (MVB) formation, cytokinesis, and viral budding. Its function is tightly regulated through interactions with cofactors like CHMP proteins and VTA1.
Antibodies targeting VPS4A are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate its expression, localization, and mechanistic roles in cellular processes. Research has linked VPS4A dysfunction to diseases, including cancer, neurodegenerative disorders (e.g., Alzheimer’s), and viral infections (e.g., HIV), due to its role in membrane dynamics and protein trafficking.
VPS4A shares homology with its paralog VPS4B, but differences in tissue-specific expression and substrate preferences necessitate isoform-specific antibodies for precise studies. Commercial VPS4A antibodies are typically validated across species (human, mouse, rat) and often characterized for cross-reactivity. Recent studies also explore its involvement in extracellular vesicle biogenesis and cellular stress responses, highlighting its broader biomedical relevance. Reliable VPS4A antibodies are essential for dissecting ESCRT-mediated mechanisms and developing therapeutic strategies targeting membrane-associated pathologies.
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