| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | VDAC1; PORIN; PORIN-31-HL |
| Entrez GeneID | 7416 |
| WB Predicted band size | Calculated MW: 31 kDa; Observed MW: 31 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human VDAC1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于VDAC1抗体的3篇参考文献示例(信息基于公开研究总结，非真实文献)：
1. **文献名称**：*VDAC1 Antibody-Based Analysis of Mitochondrial Membrane Permeability Transition in Neurodegenerative Diseases*
**作者**：Smith J et al.
**摘要**：研究使用特异性VDAC1抗体检测阿尔茨海默病模型中线粒体膜通道的变化，发现VDAC1寡聚化增加与神经元凋亡相关，提示其作为疾病生物标志物的潜力。
2. **文献名称**：*Role of VDAC1 in Cancer Metabolism: Antibody-Mediated Knockdown Suppresses Tumor Growth*
**作者**：Chen L et al.
**摘要**：通过VDAC1抗体进行免疫印迹和免疫荧光实验，验证其在癌细胞线粒体中的高表达，并证明靶向VDAC1可抑制糖代谢和肿瘤增殖，为抗癌治疗提供新思路。
3. **文献名称**：*VDAC1 Antibody Reveals Interaction with Hexokinase-II in Cardiac Ischemia*
**作者**：Garcia R et al.
**摘要**：利用VDAC1抗体进行免疫共沉淀实验，发现其与HK-II的相互作用在心肌缺血中增强，调控能量代谢失衡，为心脏保护策略提供分子机制依据。
(注：以上为模拟示例，实际文献需通过PubMed或学术数据库检索获取。)
The voltage-dependent anion channel 1 (VDAC1) antibody is a crucial tool for studying the function and expression of VDAC1. a mitochondrial outer membrane protein central to cellular energy metabolism and apoptosis regulation. VDAC1. a member of the VDAC family, forms channels that facilitate the transport of ions, nucleotides, and metabolites across the mitochondrial membrane, bridging mitochondrial and cellular metabolic activities. It also interacts with proteins like hexokinase and Bcl-2 family members, influencing apoptosis and calcium signaling.
VDAC1 antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect VDAC1 localization, expression levels, and interactions in various tissues and cell types. These antibodies help investigate VDAC1's roles in diseases, including cancer (where its dysregulation affects metabolic reprogramming), neurodegenerative disorders (e.g., Alzheimer’s and Parkinson’s linked to mitochondrial dysfunction), and cardiovascular conditions.
Researchers validate VDAC1 antibodies for specificity, often using knockout controls, to ensure accurate detection given potential cross-reactivity with VDAC2/3 isoforms. Commercial antibodies are typically raised in rabbits or mice against epitopes within VDAC1's conserved N-terminal domain. Recent studies also explore VDAC1 as a therapeutic target, with its antibodies aiding in drug development and mechanistic studies of mitochondrial-mediated cell death pathways.
×
