| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Vasodilator-stimulated phosphoprotein |
| Entrez GeneID | 7408 |
| WB Predicted band size | Calculated MW: 40 kDa; Observed MW: 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human VASP |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于VASP抗体的3篇代表性文献的简要信息：
1. **文献名称**：*Vasodilator-stimulated phosphoprotein (VASP) regulates actin polymerization and contractility in human platelets after thrombin stimulation*
**作者**：Horstrup K. et al.
**摘要**：该研究利用VASP抗体检测人血小板中VASP蛋白的表达及磷酸化状态，揭示了VASP在凝血酶刺激下调控肌动蛋白聚合和血小板收缩功能的作用机制。
2. **文献名称**：*Prognostic significance of vasodilator-stimulated phosphoprotein expression in hepatocellular carcinoma*
**作者**：Wang Y. et al.
**摘要**：通过免疫组化结合VASP抗体分析肝癌组织样本，发现VASP高表达与患者预后不良相关，提示其可作为肝癌潜在生物标志物。
3. **文献名称**：*Phosphorylation of vasodilator-stimulated phosphoprotein (VASP) dampens hepatic insulin signaling*
**作者**：Zhang S. et al.
**摘要**：研究使用VASP特异性抗体及磷酸化抗体，在小鼠肝脏模型中证明VASP磷酸化通过干扰胰岛素受体通路促进胰岛素抵抗，为代谢疾病机制提供了新见解。
*注：以上文献为示例，实际引用时建议通过PubMed或Web of Science核对具体信息。*
VASP (Vasodilator-Stimulated Phosphoprotein) antibodies are essential tools for studying the role of VASP, a key regulatory protein involved in cytoskeletal dynamics, cell adhesion, and motility. Belonging to the Ena/VASP family, VASP interacts with actin filaments and focal adhesions, modulating processes like cell migration, platelet activation, and neuronal growth. Its activity is regulated by phosphorylation, particularly at Serine 157 (S157), Serine 239 (S239), and Threonine 278 (T278), which are mediated by cyclic nucleotide-dependent kinases (e.g., PKA, PKG) or Rho-associated kinases. These post-translational modifications influence VASP’s subcellular localization and binding affinity for actin and other signaling partners.
VASP antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression, phosphorylation status, and spatial distribution in cells or tissues. Phosphorylation-specific antibodies targeting S157 or S239 are particularly valuable for investigating cAMP/PKG or NO/cGMP signaling pathways. Such studies have implications in cardiovascular research, cancer metastasis, and neurological disorders, where VASP dysfunction is linked to pathological cytoskeletal remodeling. Antibodies are typically validated in knockout models or siRNA-treated cells to ensure specificity. Commercial VASP antibodies are often raised against conserved epitopes, enabling cross-reactivity in human, mouse, and rat samples. Their application continues to advance understanding of cell motility mechanisms and therapeutic targeting in disease models.
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