| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | vesicle-associated membrane protein 2 (synaptobrevin 2); SYB2; VAMP-2 |
| Entrez GeneID | 6844 |
| WB Predicted band size | Calculated MW: 13 kDa; Observed MW: 13 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human VAMP2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于VAMP2抗体的参考文献概览(基于真实研究背景，具体标题和作者可能有调整)：
1. **标题**: "VAMP2 Antibody Validation in Synaptic Vesicle Exocytosis Studies"
**作者**: Südhof TC, et al.
**摘要**: 该研究使用VAMP2特异性抗体验证了其在神经元突触囊泡中的定位，通过Western blot和免疫电镜证实抗体可特异性识别VAMP2蛋白，并应用于囊泡分泌机制的功能性研究。
2. **标题**: "Subcellular Localization of VAMP2 in Neurons Using a Novel Monoclonal Antibody"
**作者**: Takamori S, et al.
**摘要**: 报道了一种高特异性单克隆VAMP2抗体的开发，通过免疫荧光和共聚焦显微镜观察到VAMP2在突触前末梢的聚集分布，并证明其在突触传递中的关键作用。
3. **标题**: "Antibody-Based Inhibition of VAMP2 Proteolysis Reveals Its Role in Neurotransmitter Release"
**作者**: Jahn R, et al.
**摘要**: 利用VAMP2抗体阻断蛋白酶对VAMP2的切割，证明该蛋白的完整性对钙离子触发的神经递质释放至关重要，揭示了其与SNARE复合体功能的直接关联。
**备注**：以上文献为示例性质，实际引用需根据具体论文调整。建议通过PubMed或Google Scholar搜索关键词“VAMP2 antibody Western blot/immunofluorescence”获取最新研究。
VAMP2 (vesicle-associated membrane protein 2), also known as synaptobrevin-2. is a key component of the SNARE complex that mediates synaptic vesicle fusion and neurotransmitter release in neurons. As a transmembrane protein localized to synaptic vesicles, VAMP2 interacts with plasma membrane SNAREs (syntaxin and SNAP-25) to drive the exocytotic machinery critical for neuronal communication. Antibodies targeting VAMP2 are essential tools for investigating synaptic function, vesicle trafficking, and neurological disorders associated with synaptic dysfunction.
These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to map VAMP2 expression patterns in brain tissues or cultured neurons. Researchers employ VAMP2 antibodies to study synaptic density alterations in neurodegenerative diseases (e.g., Alzheimer's, Parkinson's), psychiatric disorders, and epilepsy. Specificity is crucial due to homology with other VAMP isoforms (VAMP1/VAMP3), requiring validation through knockout controls. Some studies also explore VAMP2's role in non-neuronal secretory pathways, including hormone release and immune cell function. Recent applications extend to super-resolution microscopy and single-molecule tracking to visualize vesicle dynamics. As synaptic biomarkers gain traction in neurological research, VAMP2 antibodies remain vital for understanding both basic neurobiology and disease mechanisms.
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