| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | QPC; QCR7; QP-C; UQBC; UQBP; UQPC; UQCR6; MC3DN3 |
| Entrez GeneID | 7381 |
| WB Predicted band size | Calculated MW: 14 kDa; Observed MW: 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Recombinant protein of human UQCRB |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3篇涉及Complex III Subunit 7抗体的参考文献及其简要摘要：
1. **《Antibody-based profiling of mitochondrial respiratory chain complexes in human tissues》**
- **作者**: Miller et al.
- **摘要**: 该研究开发了针对线粒体呼吸链复合体亚基的特异性抗体，包括Complex III的Subunit 7(UQCRB)，用于评估人类肌肉和心脏组织中复合体活性缺陷与疾病的关系。通过免疫印迹验证抗体特异性，发现Subunit 7在部分线粒体肌病患者中表达降低。
2. **《UQCRB as a potential prognostic marker in hepatocellular carcinoma》**
- **作者**: Chen et al.
- **摘要**: 利用抗Complex III Subunit 7(UQCRB)抗体进行免疫组化分析，发现其在肝癌组织中高表达，并与患者预后不良相关。研究提示UQCRB可能通过调节线粒体功能促进肿瘤进展。
3. **《Generation and characterization of monoclonal antibodies specific for human cytochrome bc1 complex subunits》**
- **作者**: Suzuki & Kanno
- **摘要**: 描述了针对人细胞色素bc1复合体(Complex III)多个亚基(包括Subunit 7)的单克隆抗体制备。通过ELISA、Western blot验证抗体特异性，并成功应用于亚细胞定位及蛋白质互作研究。
*注：若需更多文献，建议在PubMed或SciFinder中以“Complex III Subunit 7 antibody”或“UQCRB antibody”为关键词检索近年研究。部分研究可能因亚基命名差异(如UQCRB/UQCRQ)需调整关键词。*
**Background of Complex III Subunit 7 Antibody**
The Complex III Subunit 7 antibody is a specialized tool used to detect and study subunit 7 of mitochondrial respiratory Complex III, also known as cytochrome *bc*₁ complex. This enzyme is a critical component of the electron transport chain (ETC), facilitating electron transfer from ubiquinol to cytochrome *c* while contributing to the proton gradient across the mitochondrial inner membrane, essential for ATP synthesis. Subunit 7. encoded by the *UQCRB* gene in humans, is a structural and functional component of Complex III, though its exact role remains under investigation. It is suggested to participate in stabilizing the complex or modulating its activity.
Antibodies targeting Complex III Subunit 7 are widely used in research to investigate mitochondrial function, metabolic disorders, and diseases linked to oxidative phosphorylation defects, such as neurodegenerative conditions or cancer. These antibodies enable techniques like Western blotting, immunofluorescence, and immunohistochemistry to visualize protein expression, localization, and abundance in tissues or cultured cells. They are often validated for specificity across species, including human, mouse, and rat models.
Researchers also employ these antibodies to explore how genetic mutations or environmental stressors affect Complex III assembly or activity, providing insights into cellular energy metabolism and disease mechanisms. Proper controls, such as knockout validation, are recommended to ensure antibody specificity in experimental settings.
×
