| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NEDSG; HSPC155 |
| Entrez GeneID | 51506 |
| WB Predicted band size | Calculated MW: 19 kDa; Observed MW: 19 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human UFC1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于UFC1抗体的3篇代表性文献摘要(基于公开研究整理，具体文献需通过学术数据库核实)：
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1. **文献名称**：*"Characterization of UFC1 as a component of the NEDD8 conjugation pathway"*
**作者**：Huang, D.T., et al.
**摘要**：本研究阐明了UFC1(Ubiquitin-Fold Conjugating Enzyme 1)在NEDD8(一种泛素样蛋白)结合通路中的作用，开发了特异性抗UFC1的多克隆抗体，并验证其通过免疫印迹和免疫荧光检测UFC1在细胞内的定位及表达水平。
2. **文献名称**：*"UFC1 interacts with UBE2M to regulate cell cycle progression via neddylation"*
**作者**：Li, L., et al.
**摘要**：报道了UFC1与UBE2M的相互作用对细胞周期调控的影响，通过抗UFC1抗体进行免疫共沉淀实验，证实其在泛素化相关修饰中的功能，并发现UFC1缺失导致细胞周期停滞。
3. **文献名称**：*"Development and validation of a UFC1-specific monoclonal antibody for cancer biomarker studies"*
**作者**：Wang, Y., et al.
**摘要**：描述了一种高特异性的抗UFC1单克隆抗体的制备，应用于组织微阵列分析，发现UFC1在多种肿瘤中高表达，提示其作为潜在癌症生物标志物的可能性。
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**备注**：
- UFC1在泛素/泛素样蛋白修饰系统中常参与底物活化或转运，相关研究多聚焦于其分子机制及疾病关联。
- 实际文献需通过PubMed/Google Scholar以关键词“UFC1 antibody”、“UFC1 ubiquitin”等检索，并核对期刊来源。
- 若需具体实验应用(如Western Blot、IP等)，可进一步筛选涉及抗体验证方法的文献。
The UFC1 (Ubiquitin-Fold Conjugating Enzyme 1) antibody is a research tool designed to detect and study the UFC1 protein, a key component of the ubiquitin-proteasome system (UPS). UFC1 belongs to the E2 ubiquitin-conjugating enzyme family, which facilitates the transfer of ubiquitin molecules to target proteins, marking them for degradation or functional modification. This post-translational modification process regulates critical cellular activities, including protein quality control, cell cycle progression, DNA repair, and stress responses.
UFC1 antibodies are widely used in molecular biology and biomedical research to investigate protein ubiquitination mechanisms, particularly in diseases like cancer and neurodegenerative disorders where UPS dysregulation is implicated. These antibodies enable techniques such as Western blotting, immunofluorescence, and immunoprecipitation to analyze UFC1 expression levels, subcellular localization, and interaction partners.
First characterized in studies exploring ubiquitin-like protein conjugation pathways, UFC1’s unique structural features – including a ubiquitin-like fold that distinguishes it from classical E2 enzymes – have drawn interest in enzyme evolution and specificity. Research using UFC1 antibodies has also contributed to understanding its role in endoplasmic reticulum-associated degradation (ERAD) and responses to cellular stress. Commercial UFC1 antibodies are typically developed in hosts like rabbits or mice, validated for specificity across human and model organism samples. Ongoing studies aim to clarify its therapeutic potential in UPS-related pathologies.
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