| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | UBA2; SAE2; UBLE1B; HRIHFB2115; SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B |
| Entrez GeneID | 10054 |
| WB Predicted band size | Calculated MW: 71 kDa; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human UBA2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于UBA2抗体的3篇参考文献示例(内容基于虚构研究概括，仅供参考)：
1. **文献名称**：*"UBA2 promotes tumorigenesis in colorectal cancer via MYC signaling activation"*
**作者**：Wang et al.
**摘要**：本研究通过UBA2抗体检测发现，UBA2在结直肠癌组织中高表达，并与患者预后不良相关。机制研究表明，UBA2通过激活MYC信号通路促进肿瘤细胞增殖，其缺失可抑制体内外肿瘤生长。
2. **文献名称**：*"UBA2-mediated SUMOylation regulates DNA damage response in breast cancer cells"*
**作者**：Huang et al.
**摘要**：利用UBA2抗体进行免疫沉淀分析，作者发现UBA2通过SUMO化修饰调控乳腺癌细胞中DNA损伤应答蛋白(如RNF4)的稳定性，抑制UBA2会导致同源重组修复缺陷并增强放疗敏感性。
3. **文献名称**：*"Functional characterization of UBA2 in SUMO activation using CRISPR-Cas9 and antibody-based knockdown models"*
**作者**：Liang et al.
**摘要**：通过UBA2抗体结合功能缺失实验，证实UBA2是SUMO化级联反应的核心组分，其缺失导致细胞周期停滞及HSP70等关键蛋白的SUMO修饰异常，进而引发细胞凋亡。
注：以上文献为示例，实际引用需查询PubMed或Google Scholar获取真实研究。
The UBA2 antibody is a crucial tool in studying the ubiquitin-like modifier-activating enzyme 2 (UBA2), a key component of the SUMO (Small Ubiquitin-like Modifier) activation pathway. UBA2. along with SAE1. forms the heterodimeric E1 enzyme responsible for initiating SUMOylation—a post-translational modification process regulating diverse cellular functions, including protein localization, DNA repair, and transcriptional regulation. By catalyzing ATP-dependent SUMO activation and its transfer to E2 conjugating enzymes, UBA2 plays a pivotal role in maintaining genomic stability, cell cycle progression, and stress responses.
Antibodies targeting UBA2 enable researchers to investigate its expression, interactions, and regulatory mechanisms in both normal and pathological contexts. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to assess protein levels, subcellular localization, and post-translational modifications. UBA2 dysregulation has been implicated in cancers, neurodegenerative diseases, and viral infections, making its antibody a valuable reagent for exploring disease mechanisms and therapeutic targets. Additionally, studies using UBA2 antibodies have shed light on its role in SUMO paralog-specific pathways (SUMO-1. SUMO-2/3) and crosstalk with ubiquitination. Validation of these antibodies often includes knockout cell lines or siRNA knockdown to ensure specificity, given the structural similarities within E1 enzyme families. Overall, UBA2 antibodies are indispensable for unraveling the complexities of SUMOylation and its impact on cellular homeostasis and disease.
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