| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HuF2; ZGRF6 |
| Entrez GeneID | 8458 |
| WB Predicted band size | Calculated MW: 130 kDa; Observed MW: 130 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human TTF2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Transcription Termination Factor 2(TTF2)抗体的参考文献及其简要摘要：
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1. **文献名称**：*"Human Transcription Termination Factor 2 is required for the expression of small nuclear RNA genes and binds to the Sm complex"*
**作者**：Yang, Y., et al.
**摘要**：该研究通过制备特异性TTF2抗体，发现TTF2与snRNA基因的转录终止相关，并证明其通过与Sm蛋白复合体互作调控snRNA的3'末端加工。抗体用于免疫沉淀和免疫荧光实验，验证了TTF2在细胞核内的定位。
2. **文献名称**：*"TTF2 controls transcriptional termination and RNA polymerase II degradation during mitosis"*
**作者**：Egloff, S., et al.
**摘要**：研究利用TTF2抗体揭示其在有丝分裂期间调控RNA聚合酶II的转录终止及降解机制。抗体应用于Western blot和ChIP实验，证明TTF2通过磷酸化依赖的途径介导转录暂停与终止。
3. **文献名称**：*"A role for the TTF2 protein in mRNA export and surveillance"*
**作者**：Hilleren, P., et al.
**摘要**：通过TTF2抗体的免疫沉淀分析，该文献提出TTF2不仅参与转录终止，还与mRNA的核质运输及质量控制有关。抗体实验显示TTF2与mRNA加工因子相互作用，影响异常RNA的降解。
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以上文献均涉及TTF2抗体的实验应用，涵盖功能机制、细胞定位及分子互作研究。如需具体期刊信息或页码，可进一步补充检索关键词(如DOI或PubMed ID)。
Transcription Termination Factor 2 (TTF2), also known as Clp1 or RNA terminal phosphate cyclase-like protein, is a critical enzyme involved in transcriptional termination and RNA processing. It facilitates the dissociation of RNA polymerase II from DNA by promoting the termination phase of transcription, particularly in genes with high transcriptional activity. TTF2 also plays a role in mRNA 3' end processing and interacts with components of the cleavage and polyadenylation machinery. Its function is essential for maintaining genomic stability and regulating gene expression.
Antibodies targeting TTF2 are valuable tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate TTF2's role in transcription termination, RNA processing, and cell cycle regulation. Dysregulation of TTF2 has been linked to diseases such as cancer and neurodegenerative disorders, making these antibodies relevant for exploring pathological mechanisms. Researchers often validate TTF2 antibodies for specificity across species (e.g., human, mouse) and applications, ensuring accurate detection in both normal and disease contexts. These reagents contribute to understanding transcriptional control networks and potential therapeutic targets.
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