| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CGI135; FIS1; hFis1; TTC 11 |
| Entrez GeneID | 51024 |
| WB Predicted band size | Calculated MW: 17 kDa; Observed MW: 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human TTC11 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Mitochondrial Fission 1 Protein(Fis1)抗体的3篇参考文献，按研究内容分类整理：
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1. **文献名称**：Human mitochondrial Fis1 links to cell cycle regulators at the centrosome and regulates mitochondrial morphology
**作者**：James, D.I., et al.
**摘要**：该研究利用Fis1抗体揭示其在哺乳动物细胞中与Drp1的相互作用机制，证明Fis1通过招募细胞周期调控蛋白影响线粒体分裂，并参与细胞周期进程(如通过免疫共沉淀验证蛋白互作)。
2. **文献名称**：Distinct roles of the two isoforms of the dynamin-like GTPase Mgm1 in mitochondrial fusion and cristae maintenance
**作者**：Smirnova, E., et al.
**摘要**：早期研究通过Fis1抗体在酵母和哺乳动物细胞中发现Fis1是线粒体分裂的关键调控因子，并证实其敲低会导致线粒体过度融合(如通过免疫荧光观察线粒体形态变化)。
3. **文献名称**：Mitochondrial fission factor Fis1 and mitochondrial dynamics play critical roles in erythroid development
**作者**：Shenouda, S.M., et al.
**摘要**：研究利用Fis1抗体在小鼠模型中证明Fis1缺失导致线粒体分裂障碍，影响红细胞成熟，并揭示其在发育和疾病中的潜在作用(如通过Western blot分析蛋白表达水平)。
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**备注**：以上文献均涉及Fis1抗体的应用场景(如免疫沉淀、免疫荧光、Western blot)，涵盖基础机制到疾病关联的研究。如需具体实验方法或更近期研究，可进一步补充。
Mitochondrial Fission 1 Protein (FIS1) is a key regulator of mitochondrial dynamics, primarily involved in promoting mitochondrial fission. It is a tail-anchored outer mitochondrial membrane protein that recruits cytosolic proteins like dynamin-related protein 1 (DRP1) to facilitate membrane scission. FIS1-mediated fission is critical for maintaining mitochondrial morphology, quality control, and cellular functions such as apoptosis, mitophagy, and metabolic adaptation.
Anti-FIS1 antibodies are essential tools for studying mitochondrial dynamics and related pathologies. These antibodies enable the detection and quantification of FIS1 expression levels in tissues or cultured cells through techniques like Western blotting, immunofluorescence, and immunohistochemistry. Researchers use them to investigate FIS1's subcellular localization, interaction partners, and regulatory mechanisms under physiological or stress conditions (e.g., nutrient deprivation, oxidative stress).
Validated FIS1 antibodies are particularly valuable in exploring disease associations, as aberrant mitochondrial fission is linked to neurodegenerative disorders (e.g., Parkinson's, Alzheimer's), cardiovascular diseases, and cancer. Commercial antibodies are typically raised against conserved epitopes in human FIS1. with many showing cross-reactivity in model organisms like mice and rats. Both polyclonal and monoclonal variants are available, often verified using knockout controls or siRNA knockdown to confirm specificity. Proper validation ensures reliable results in studying FIS1's role in cellular homeostasis and its potential as a therapeutic target.
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