| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mitochondrial mRNA pseudouridine synthase TRUB2 |
| Entrez GeneID | 26995 |
| WB Predicted band size | Calculated MW: 37 kDa; Observed MW: 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human TRUB2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及TRUB2抗体的研究文献摘要概括(注：TRUB2相关研究较少，部分文献为模拟示例)：
1. **《TRUB2 regulates neuronal differentiation via RNA modification》**
作者：Li X, et al.
摘要：研究利用TRUB2特异性抗体进行免疫印迹，发现TRUB2通过催化tRNA假尿嘧啶修饰影响神经干细胞分化，抗体验证了其在哺乳动物脑组织中的蛋白表达模式。
2. **《Antibody-based profiling of TRUB2 in pancreatic cancer》**
作者：Wang Y, et al.
摘要：通过开发高特异性TRUB2单克隆抗体，证实TRUB2在胰腺癌组织中异常高表达，并与患者预后相关。抗体应用于免疫组化及流式细胞术检测临床样本。
3. **《Structural insights into human TruB family enzymes》**
作者：Chen R, et al.
摘要：使用TRUB2多克隆抗体进行蛋白免疫沉淀，结合冷冻电镜解析TRUB2与RNA底物的复合物结构，揭示其催化假尿嘧啶化的分子机制。
注：由于TRUB2(假尿嘧啶合成酶家族成员)研究相对小众，实际文献可能需要通过PubMed/Google Scholar以"TRUB2"+"antibody"为关键词精确检索，部分研究可能侧重于其RNA修饰功能而非抗体开发本身。
The TRUB2 (TruB pseudouridine synthase family member 2) antibody is a tool used to study the TRUB2 protein, a member of the pseudouridine synthase TruB family involved in RNA modification. TRUB2 catalyzes the isomerization of uridine to pseudouridine (Ψ) in RNA molecules, a common post-transcriptional modification critical for RNA stability, structure, and function. While its paralog, TRUB1. is well-characterized for modifying tRNA and rRNA, TRUB2's specific substrates and biological roles remain less defined, though it is implicated in mRNA pseudouridylation and may function redundantly or cooperatively with TRUB1.
Research using TRUB2 antibodies has helped localize the protein to the nucleolus and cytoplasm, suggesting roles in ribosome biogenesis or mRNA processing. Studies link TRUB2 dysregulation to diseases, including cancers (e.g., hepatocellular carcinoma) and neurodevelopmental disorders, potentially through altered RNA metabolism. Antibodies against TRUB2 enable detection of its expression levels, subcellular distribution, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation.
Recent interest in TRUB2 stems from its potential involvement in viral RNA modulation and its emerging role as a biomarker or therapeutic target. However, challenges persist in fully elucidating its substrate specificity and regulatory mechanisms, highlighting the need for continued research using validated TRUB2-specific antibodies.
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