| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAT2; ECAC1; OTRPC3 |
| Entrez GeneID | 56302 |
| WB Predicted band size | Calculated MW: 83 kDa; Observed MW: 83 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human TRPV5 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于TrpV5抗体的3篇示例参考文献(内容为模拟概括，仅供参考)：
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1. **文献名称**: "Immunolocalization and functional characterization of the calcium-selective channel TRPV5 in the kidney"
**作者**: Hoenderop JG, et al.
**摘要**: 该研究通过特异性TrpV5抗体定位了该通道蛋白在小鼠肾脏远曲小管上皮细胞的表达，证实其在钙离子重吸收中的关键作用，并通过抗体阻断实验验证了TrpV5对钙离子通透性的调控功能。
2. **文献名称**: "Regulation of TRPV5 by pH and protein kinase C in renal epithelial cells"
**作者**: Peng JB, et al.
**摘要**: 利用TrpV5抗体进行Western blot和免疫荧光分析，揭示了细胞外pH变化和PKC信号通路对TrpV5蛋白表达及膜定位的调控机制，为理解钙代谢异常相关疾病提供分子基础。
3. **文献名称**: "TRPV5 deficiency induces calcium malabsorption and bone loss in mice"
**作者**: van der Eerden BC, et al.
**摘要**: 通过TrpV5基因敲除小鼠模型及抗体免疫组化分析，证明TrpV5缺失导致肠道和肾脏钙吸收减少，引发继发性骨质疏松，提示其作为治疗骨代谢疾病的潜在靶点。
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*注：以上文献为示例性内容，实际引用需通过PubMed、Google Scholar等数据库检索真实文献。建议使用关键词“TRPV5 antibody”或“TRPV5 immunohistochemistry”进行精确查询。*
The Transient Receptor Potential Vanilloid 5 (TrpV5) antibody is a critical tool for studying the TrpV5 calcium channel, a protein pivotal in calcium (Ca²⁺) reabsorption and homeostasis. TrpV5. also known as epithelial calcium channel ECaC1. is predominantly expressed in the kidney's distal convoluted tubules, where it facilitates Ca²⁺ uptake from urine into cells, a process vital for maintaining systemic calcium balance. Dysregulation of TrpV5 is linked to disorders like hypercalciuria, kidney stones, and osteoporosis, driving research interest in its function and regulation.
TrpV5 antibodies are designed to target specific epitopes of the channel, enabling detection, localization, and quantification in tissues or cell lines via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help investigate TrpV5's expression patterns, post-translational modifications (e.g., glycosylation), and interactions with regulatory proteins like calmodulin or klotho.
Structurally, TrpV5 features six transmembrane domains with intracellular N- and C-termini, distinguishing it from other TRP channels. Its activity is modulated by pH, hormones (e.g., vitamin D, parathyroid hormone), and dietary calcium. Research using TrpV5 antibodies has elucidated its role in hypercalciuric diseases, renal dysfunction, and its crosstalk with TrpV6. a related channel in intestinal calcium absorption.
Commercial TrpV5 antibodies are typically validated for specificity using knockout models or siRNA silencing. Their applications extend to translational studies, including drug development for calcium-related pathologies and genetic disorders like familial hypocalciuric hypercalcemia.
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