| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C11orf15 |
| Entrez GeneID | 56674 |
| WB Predicted band size | Calculated MW: 23 kDa; Observed MW: 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human TMEM9B |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Transmembrane Protein 9B(TMEM9B)抗体的模拟参考文献示例，供参考：
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1. **文献名称**：*TMEM9B interacts with vesicular ATPase to regulate lysosomal acidification and Wnt signaling*
**作者**：Zhang L, et al.
**摘要**：本研究利用特异性TMEM9B抗体进行Western blot和免疫荧光分析，揭示了TMEM9B通过与v-ATPase相互作用调控溶酶体酸化，进而影响Wnt/β-catenin信号通路的分子机制。
2. **文献名称**：*Overexpression of TMEM9B in colorectal cancer correlates with poor prognosis*
**作者**：Chen Y, et al.
**摘要**：通过免疫组化(使用抗人TMEM9B多克隆抗体)分析结直肠癌组织样本，发现TMEM9B高表达与患者预后不良显著相关，提示其作为潜在生物标志物的价值。
3. **文献名称**：*Development of a novel monoclonal antibody targeting human TMEM9B for functional studies*
**作者**：Wang X, et al.
**摘要**：本文报道了一种新型抗TMEM9B单克隆抗体的开发与验证，该抗体在流式细胞术、免疫沉淀等实验中表现出高特异性和敏感性，适用于多种细胞模型的功能研究。
4. **文献名称**：*TMEM9B modulates Notch signaling via lysosome-mediated protein degradation*
**作者**：Kim S, et al.
**摘要**：利用TMEM9B抗体进行免疫电镜和共聚焦成像，发现其在溶酶体定位的调控作用，并进一步阐明其对Notch信号通路中关键蛋白稳定性的影响。
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**注**：以上文献为模拟生成，实际引用时请通过PubMed、Web of Science等平台检索真实发表的论文，并核实抗体应用的具体细节(如货号、物种反应性等)。
Transmembrane Protein 9B (TMEM9B) is a conserved protein localized to cellular membranes, notably lysosomes and endosomes, where it regulates organelle acidification and vesicular trafficking. Studies suggest it interacts with vacuolar ATPase (V-ATPase), a key complex for maintaining pH gradients, influencing lysosomal function, nutrient sensing, and autophagy. TMEM9B is implicated in cancer progression, with elevated expression observed in certain tumors, potentially promoting proliferation, metastasis, or drug resistance.
TMEM9B antibodies are critical tools for studying its expression, localization, and functional roles. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers use these antibodies to explore TMEM9B’s involvement in diseases, particularly cancer and lysosomal storage disorders. However, challenges persist in confirming antibody specificity due to TMEM9B’s hydrophobic transmembrane domains and potential cross-reactivity with homologous proteins. Recent studies also investigate its interaction with signaling pathways (e.g., Wnt, mTOR) and its role in viral entry mechanisms. Despite progress, the full molecular mechanism of TMEM9B remains understudied, necessitating further validation of antibody reliability and functional assays.
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