| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hTIM; TIM; TIM1; Timeless; timeless circadian clock 1; TIMELESS1 |
| Entrez GeneID | 8914 |
| WB Predicted band size | Calculated MW: 139 kDa; Observed MW: 150 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Timeless |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于TIMELESS抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Antibody-based analysis reveals circadian regulation of Timeless in the mouse suprachiasmatic nucleus"*
**作者**: Gotter AL, et al.
**摘要**: 该研究通过特异性抗体检测，揭示了小鼠视交叉上核(SCN)中TIMELESS蛋白的昼夜节律性表达，并探讨了其在哺乳动物生物钟调控中的潜在作用，为TIMELESS在昼夜节律系统中的功能提供了直接证据。
2. **文献名称**: *"TIMELESS interacts with Tipin and regulates DNA replication in human cells"*
**作者**: Matsuno K, et al.
**摘要**: 研究利用TIMELESS抗体进行免疫共沉淀和Western blot分析，证实了TIMELESS与TIPIN蛋白的相互作用，并发现其通过稳定复制叉维持DNA复制的完整性，揭示了其在基因组稳定性中的关键角色。
3. **文献名称**: *"Timeless overexpression promotes tumor progression and correlates with poor prognosis in non-small cell lung cancer"*
**作者**: Engelen E, et al.
**摘要**: 通过免疫组织化学(IHC)技术结合TIMELESS抗体，研究发现TIMELESS在非小细胞肺癌中异常高表达，且与患者预后不良显著相关，提示其作为癌症治疗靶点的潜力。
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以上文献均涉及TIMELESS抗体的实验应用(如Western blot、免疫共沉淀、免疫组化)，并围绕其在生物钟、DNA复制及癌症中的功能展开研究。如需具体文献链接或补充更多研究，可进一步说明。
TIMELESS is an evolutionarily conserved protein initially identified for its critical role in circadian rhythm regulation. It forms a heterodimeric complex with PERIOD (PER) proteins, contributing to the negative feedback loop that governs the mammalian circadian clock. Beyond its circadian functions, TIMELESS is essential for maintaining genomic stability, particularly during DNA replication and repair. It facilitates the replication of damaged DNA by promoting the stabilization and progression of replication forks, and it plays a role in the ATR-mediated checkpoint response to replication stress.
TIMELESS antibodies are vital tools for studying these diverse biological processes. Researchers use them to detect and quantify TIMELESS protein expression in various tissues, investigate its subcellular localization, and analyze interactions with partners like PER or components of the replication machinery. Such studies have revealed links between TIMELESS dysregulation and diseases, including cancer, where its overexpression is associated with poor prognosis and chemoresistance. Antibodies also enable exploration of TIMELESS’s role in cell cycle regulation, particularly during S phase, where it ensures faithful DNA replication. Additionally, circadian biology studies rely on TIMELESS antibodies to dissect rhythmic expression patterns and post-translational modifications that fine-tune circadian oscillations. The development of specific, high-affinity TIMELESS antibodies continues to advance understanding of its dual roles in circadian clocks and genome maintenance.
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