| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | T box 3; T-box protein 3; Tbx3; TBX3 ISO; UMS; XHL |
| Entrez GeneID | 6926 |
| WB Predicted band size | Calculated MW: 79 kDa; Observed MW: 79 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | A synthetic peptide of human Tbx3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于 **T-box Protein 3 (TBX3)** 抗体的参考文献示例，按研究方向和内容概括整理：
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1. **文献名称**：*TBX3 promotes human breast cancer cells invasion and migration via regulating Wnt/β-catenin pathway*
**作者**：Lin et al. (2018)
**摘要**：通过免疫组化(IHC)和Western blot分析，研究TBX3抗体在乳腺癌组织中的高表达及其促进癌细胞侵袭转移的机制，发现其通过激活Wnt/β-catenin信号通路发挥作用。
2. **文献名称**：*The role of TBX3 in embryonic development and disease*
**作者**：Hoogaars et al. (2019)
**摘要**：综述TBX3在胚胎心脏和四肢发育中的关键调控作用，利用抗体染色技术(如免疫荧光)揭示其表达模式异常与先天性疾病的关联。
3. **文献名称**：*TBX3 expression correlates with poor prognosis in melanoma*
**作者**：Rodriguez-Seguel et al. (2020)
**摘要**：使用TBX3抗体进行组织芯片分析，发现黑色素瘤中TBX3过表达与患者生存率下降相关，并证实其通过抑制p53通路促进肿瘤进展。
4. **文献名称**：*Dual role of TBX3 in cancer: Oncogene and tumor suppressor*
**作者**：Kumar et al. (2017)
**摘要**：结合多种抗体检测手段(如流式细胞术)，探讨TBX3在不同癌症中的双重功能，强调其表达水平与组织类型和微环境信号(如TGF-β)的关联。
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**注**：以上为示例性内容，实际文献需通过PubMed、Google Scholar等平台检索确认具体信息。若需特定研究方向的文献，可进一步补充关键词筛选。
The T-box protein 3 (TBX3) is a member of the T-box family of transcription factors, characterized by a conserved DNA-binding domain called the T-box. TBX3 plays critical roles in embryonic development, particularly in regulating organogenesis, limb formation, and cell fate determination. It is known to repress transcription of target genes, including the tumor suppressor p14ARF (in mice) or p19ARF (in humans), thereby influencing cell proliferation, differentiation, and apoptosis. Dysregulation of TBX3 has been implicated in developmental disorders and cancers. For example, mutations in TBX3 are linked to ulnar-mammary syndrome, a congenital condition affecting limb, apocrine gland, and genital development. In cancer, TBX3 overexpression is associated with tumor progression, metastasis, and drug resistance in melanoma, breast, and liver cancers.
Antibodies targeting TBX3 are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies are typically generated using immunogens derived from specific regions of the human or murine TBX3 protein, such as the N-terminal or T-box domain. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to investigate TBX3's role in gene regulation, developmental pathways, and oncogenic mechanisms. Validation of TBX3 antibodies includes testing for specificity via knockout cell lines or siRNA-mediated knockdown. Researchers also utilize these antibodies to explore TBX3's potential as a diagnostic marker or therapeutic target, particularly in cancers where its aberrant expression correlates with poor prognosis.
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