| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | STAU; PPP1R150 |
| Entrez GeneID | 6780 |
| WB Predicted band size | Calculated MW: 63 kDa; Observed MW: 63 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Staufen |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及Staufen抗体的参考文献及其摘要概览：
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1. **文献名称**：*Staufen protein associates with the 3'UTR of bicoid mRNA to form particles that move in a microtubule-dependent manner*
**作者**：St Johnston, D., Beuchle, D., & Nüsslein-Volhard, C. (1991)
**摘要**：该研究首次在果蝇中鉴定了Staufen蛋白，并利用特异性抗体通过免疫荧光技术揭示了Staufen与bicoid mRNA的3'非翻译区结合，形成颗粒并通过微管依赖性机制在胚胎中定位，为RNA运输机制提供了关键证据。
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2. **文献名称**：*Staufen1 is a component of RNA granules in mammalian cells and regulates dendritic mRNA trafficking*
**作者**：Duchaine, T.F., et al. (2002)
**摘要**：本文通过哺乳动物细胞中Staufen1抗体的免疫沉淀和共聚焦显微镜技术，证明Staufen1参与RNA颗粒的组装，并调控树突状细胞中mRNA的运输，提示其在神经元功能中的潜在作用。
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3. **文献名称**：*Staufen1-dependent dendritic targeting requires dual RNA-binding regions and occurs independently of mRNA splicing*
**作者**：Marion, R.M., et al. (2009)
**摘要**：研究利用Staufen1抗体进行免疫印迹和细胞成像，发现其通过两个RNA结合域介导树突mRNA靶向运输，且此过程不依赖RNA剪接，揭示了神经元中mRNA定位的新机制。
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这些文献均通过Staufen抗体探究了该蛋白在RNA代谢、运输及疾病中的功能，方法涵盖免疫定位、颗粒分析和功能缺失实验。如需具体实验细节，建议查阅全文。
Staufen antibodies are essential tools in studying the Staufen family of RNA-binding proteins, which play critical roles in mRNA transport, localization, and translation regulation. The Staufen proteins, first identified in *Drosophila*, are evolutionarily conserved and exist as two main isoforms in mammals: Staufen1 (STAU1) and Staufen2 (STAU2). These proteins bind double-stranded RNA structures within target mRNAs, facilitating their transport to specific subcellular regions, such as neuronal dendrites, where localized protein synthesis supports synaptic plasticity and memory formation.
Staufen1 is primarily cytoplasmic and involved in mRNA decay pathways, including Staufen-mediated decay (SMD), while Staufen2 is enriched in neurons and associated with dendritic RNA granules, influencing synaptic function. Antibodies targeting Staufen proteins enable researchers to investigate their expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Such studies have linked Staufen dysregulation to neurological disorders, including Alzheimer’s disease and fragile X syndrome, as well as viral infections (e.g., HIV), where Staufen1 interacts with viral RNAs.
The development of isoform-specific antibodies has been crucial for distinguishing the unique and overlapping roles of Staufen1/2 in RNA metabolism and cellular homeostasis. Their application in disease models continues to shed light on post-transcriptional regulatory mechanisms, offering potential therapeutic insights.
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