| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HBP |
| Entrez GeneID | 7884 |
| WB Predicted band size | Calculated MW: 31 kDa; Observed MW: 31 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human SLBP |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于SLBP抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: *Purification and Characterization of the Stem-Loop Binding Protein (SLBP) Involved in Histone mRNA 3' End Processing*
**作者**: Wang, Z.F., et al.
**摘要**: 该研究报道了SLBP的纯化及多克隆抗体的制备，证实SLBP特异性结合组蛋白mRNA的茎环结构，并参与其3'端加工。抗体用于检测SLBP在细胞周期中的表达变化及核内定位。
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2. **文献名称**: *Role of SLBP in Cell Cycle-Regulated Histone mRNA Turnover*
**作者**: Whitfield, M.L., et al.
**摘要**: 通过SLBP抗体进行免疫沉淀和功能抑制实验，揭示了SLBP在S期组蛋白mRNA稳定性和细胞周期依赖性降解中的核心作用，证明其缺失导致mRNA异常积累。
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3. **文献名称**: *SLBP Cooperates with RNA-Binding Proteins in DNA Damage Response*
**作者**: Sullivan, K.D., et al.
**摘要**: 研究利用SLBP抗体进行免疫荧光和Western blot分析，发现DNA损伤后SLBP与RNA结合蛋白(如HuR)相互作用，调控组蛋白mRNA代谢以维持基因组稳定性。
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**扩展参考(综述)**:
4. **文献名称**: *The Stem-Loop Binding Protein: A Key Regulator of Histone mRNA Biosynthesis*
**作者**: Marzluff, W.F., et al.
**摘要**: 综述总结了SLBP在组蛋白mRNA合成、翻译及降解中的多功能性，强调抗体工具在揭示其分子机制(如磷酸化修饰调控)中的应用。
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这些文献覆盖了SLBP抗体的开发、功能研究及在DNA损伤等领域的应用，可作为相关研究的起点。
SLBP (Stem-Loop Binding Protein) is a conserved RNA-binding protein critical for regulating histone mRNA metabolism in eukaryotes. It specifically recognizes the stem-loop structure located near the 3' end of replication-dependent histone mRNAs, which lack polyadenylated tails. SLBP plays dual roles in histone mRNA processing and translation. During the S phase of the cell cycle, it facilitates the nuclear cleavage of pre-mRNA by recruiting endonucleases and stabilizes mature histone mRNAs in the cytoplasm. Post-S phase, SLBP degradation ensures timely downregulation of histone synthesis to maintain genomic stability.
Antibodies against SLBP are essential tools for studying cell cycle regulation, histone biosynthesis, and DNA replication mechanisms. They are widely used in techniques like western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to detect SLBP expression levels, subcellular localization, and interactions with other cell cycle regulators. Dysregulation of SLBP has been linked to cancers and developmental disorders, making these antibodies valuable for investigating disease mechanisms. Commercial SLBP antibodies are typically generated using immunogenic epitopes from conserved regions (e.g., the RNA-binding domain) and validated for species cross-reactivity (human, mouse, etc.). Recent studies also explore SLBP's non-canonical roles in regulating non-histone RNAs, expanding its biological significance.
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