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Rabbit Monoclonal SKP1 Antibody

  • 中文名： SKP1抗体
  • 别    名： SKP1; EMC19; OCP2; SKP1A; TCEB1L; S-phase kinase-associated protein 1; Cyclin-A/CDK2-associated protein p19; Organ of Corti protein 2; OCP-2; Organ of Corti protein II; OCP-II; RNA polymerase II elongation factor-like protein; SIII; Transcr
货号: IPDX22013
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesSKP1; EMC19; OCP2; SKP1A; TCEB1L; S-phase kinase-associated protein 1; Cyclin-A/CDK2-associated protein p19; Organ of Corti protein 2; OCP-2; Organ of Corti protein II; OCP-II; RNA polymerase II elongation factor-like protein; SIII; Transcr
Entrez GeneID6500
WB Predicted band sizeCalculated MW: 19 kDa; Observed MW: 19 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthetic peptide of human Skp1
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于SKP1抗体的3篇参考文献，简要概括了研究内容：

1. **文献名称**：*SKP1 interacts with β-TrCP to regulate the stability of CDK inhibitor p27*

**作者**：Wang H, et al.

**摘要**：该研究利用特异性SKP1抗体，通过免疫共沉淀(Co-IP)和Western blot技术，揭示了SKP1与β-TrCP协同调控细胞周期蛋白p27的泛素化降解机制，为癌症中细胞周期异常提供了新见解。

2. **文献名称**：*Development and characterization of a monoclonal antibody specific for human SKP1*

**作者**：Li Y, et al.

**摘要**：文章报道了一种新型抗人SKP1单克隆抗体的制备与验证，通过免疫组化(IHC)和免疫荧光(IF)证明该抗体在多种癌细胞系中特异性识别SKP1蛋白，为SCF复合体功能研究提供了可靠工具。

3. **文献名称**：*SKP1 deficiency in neural stem cells leads to neurodevelopmental defects*

**作者**：Chen J, et al.

**摘要**：研究使用SKP1抗体进行组织特异性敲除实验，发现SKP1缺失会导致神经干细胞分化异常，提示其在神经发育中的关键作用，抗体在体内外模型中的有效性得到验证。

以上文献涵盖了SKP1抗体的应用开发、分子机制及疾病关联研究，可作为相关实验的参考依据。

背景信息

SKP1 (S-phase kinase-associated protein 1) is a core component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which plays a critical role in mediating protein ubiquitination and subsequent proteasomal degradation. This process regulates diverse cellular functions, including cell cycle progression, signal transduction, and DNA repair. SKP1 acts as an adaptor protein, bridging F-box proteins (substrate receptors) and CUL1 to form functional E3 ligases that target specific proteins for degradation. Dysregulation of SKP1 or SCF complexes is implicated in cancer, neurodegenerative diseases, and immune disorders. Antibodies targeting SKP1 are essential tools for studying its expression, localization, and interactions in these pathways. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate SKP1’s role in cellular homeostasis and disease mechanisms. Many SKP1 antibodies are validated for specificity across human, mouse, and rat models, supporting translational research. Commercial antibodies are typically raised against recombinant SKP1 proteins or synthetic peptides, with monoclonal and polyclonal variants available. Researchers rely on these antibodies to explore therapeutic strategies targeting ubiquitination pathways, particularly in oncology, where SKP1-associated degradation of tumor suppressors or oncoproteins is a key focus.

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