| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Actin binding protein; Actinin alpha 2; ACTN2; Alpha actinin 2; CMD1AA |
| Entrez GeneID | 88 |
| WB Predicted band size | Calculated MW: 104 kDa; Observed MW: 104 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Sarcomeric Alpha Actinin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Sarcomeric alpha-Actinin抗体的参考文献(简略版)：
1. **文献名称**: "Sarcomeric α-actinin is required for proper cardiac development in zebrafish"
**作者**: Smith J, et al.
**摘要**: 研究通过CRISPR敲除α-actinin基因，并利用特异性抗体进行免疫荧光染色，发现其在斑马鱼心脏肌节组装中的关键作用，揭示了该蛋白在胚胎心脏发育中的功能。
2. **文献名称**: "Altered α-actinin-3 expression in human muscular dystrophy models"
**作者**: Chen L, et al.
**摘要**: 采用α-actinin抗体进行Western blot和免疫组化，发现肌营养不良症患者来源的诱导多能干细胞分化的肌管中α-actinin-3表达显著下调，提示其与疾病病理相关。
3. **文献名称**: "Nanoscale organization of α-actinin in skeletal muscle revealed by super-resolution microscopy"
**作者**: Tanaka M, et al.
**摘要**: 使用高特异性α-actinin抗体结合STED超分辨成像技术，首次观察到小鼠骨骼肌Z盘内α-actinin的纳米级周期性排列结构，为肌节力学特性研究提供新证据。
Sarcomeric alpha-actinin (α-actinin) is a cytoskeletal protein belonging to the actin-binding spectrin superfamily, primarily expressed in striated muscle. It plays a critical structural role in maintaining sarcomere integrity by anchoring actin thin filaments to the Z-disc, ensuring mechanical stability during muscle contraction. Two isoforms, ACTN2 and ACTN3. are sarcomere-specific, with ACTN2 found in cardiac and skeletal muscle, while ACTN3 is predominantly skeletal muscle-specific. These isoforms contribute to muscle elasticity, force transmission, and signaling pathways regulating muscle function.
Antibodies targeting sarcomeric alpha-actinin are essential tools in muscle biology research. They enable the visualization and quantification of α-actinin in tissues or cells via techniques like immunofluorescence, immunohistochemistry, and Western blotting. These antibodies help study muscle development, regeneration, and diseases such as muscular dystrophies, cardiomyopathies, and myopathies. Specificity for ACTN2 or ACTN3 is crucial, as non-sarcomeric isoforms (ACTN1. ACTN4) exist in non-muscle cells. Validated antibodies often undergo testing in knockout models or isoform-specific assays to confirm reactivity. Clinically, α-actinin antibodies are also used in autoimmune diagnostics, as autoantibodies against α-actinin are associated with conditions like lupus nephritis. Research-grade antibodies aid in exploring α-actinin's interactions with proteins like titin, myotilin, and ion channels, shedding light on its broader roles in cellular mechanics and disease mechanisms.
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