首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠抗环胍氨酸肽抗体(Anti-CCP-antibody)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CCP Ab含量。CCP Ab即抗环瓜氨酸肽抗体，在类风湿关节炎研究中是重要靶点。它对类风湿关节炎诊断特异性高，能较早出现于疾病进程中。其研究机制主要是通过识别瓜氨酸化抗原，触发免疫反应，参与关节炎症破坏，助力疾病早期诊断与病情监测。试剂盒检测范围为3.12 IU/mL--200 IU/mL，为类风湿性关节炎等自身免疫性疾病机制研究提供高效工具；检测 Anti - CCP 抗体对疾病早期诊断及病程监测具有重要科研价值；适用于体外实验中定量评估血清、血浆等生物样本中抗体水平；科研人员可通过该产品开展疾病模型中的抗体动态变化研究、药物干预效果评价或探索 CCP 抗体与关节炎症的相关性，为免疫病理机制解析及新型治疗策略开发提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CCP Ab
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：3.12 IU/mL--200 IU/mL
灵敏度：0.78 IU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat anti-CCP-antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 85-105 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 84-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-105 | |||||
回收率：
| The recovery of rat anti-CCP-antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 83-96 | ||||
| EDTA plasma (n=4) | 94 | 85-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| IU/mlOD1OD2AverageCorrected 2002.6142.7122.6632.550 1001.9952.0122.0041.891 501.2821.2671.2751.162 250.6820.6990.6910.578 12.50.4140.4220.4180.305 6.250.2570.2560.2570.144 3.120.2020.1920.1970.084 00.1100.1150.113 | ||||||
货期：3-5 working days
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