| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1 |
| Entrez GeneID | 4893 |
| WB Predicted band size | Calculated MW: 21 kDa; Observed MW: 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human RAS |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于HRAS抗体的代表性文献(模拟示例，非真实文献)：
1. **"Monoclonal antibody Y13-259 specifically targets oncogenic HRAS in human cancers"**
*作者：Smith J et al. (2019)*
摘要：开发了单克隆抗体Y13-259.验证其对HRAS蛋白的高特异性，适用于免疫组化检测结直肠癌中HRAS突变体的过表达。
2. **"Comparative analysis of commercial HRAS antibodies for Western blot applications"**
*作者：Lee H et al. (2021)*
摘要：系统评估了5种市售HRAS抗体的灵敏度和交叉反应性，推荐克隆D12在检测GTP结合态HRAS时表现最优。
3. **"Nanobody-based HRAS activity biosensor reveals dynamic signaling in live cells"**
*作者：Gomez-Ruiz A et al. (2022)*
摘要：利用骆驼源纳米抗体构建实时监测HRAS激活状态的荧光探针，为靶向RAS信号通路药物筛选提供新工具。
*注：以上为示例性内容，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词"HRAS antibody validation"或"anti-HRAS application"筛选实验方法学相关论文。*
The HRAS antibody is a crucial tool in studying the HRAS protein, a member of the RAS family of small GTPases. HRAS functions as a molecular switch, cycling between active GTP-bound and inactive GDP-bound states to regulate signaling pathways (e.g., RAS-MAPK) that control cell proliferation, differentiation, and survival. Mutations in HRAS, particularly at codons 12. 13. or 61. lead to constitutive activation, driving uncontrolled cell growth and contributing to cancers such as thyroid carcinoma, bladder cancer, and Costello syndrome.
HRAS-specific antibodies are designed to detect endogenous HRAS protein levels, localization, and activation status. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study HRAS expression in normal vs. diseased tissues or model systems. These antibodies often distinguish between wild-type and mutant HRAS isoforms, aiding in cancer research and diagnostics. Validation typically includes testing in HRAS-knockout cells or tissues to confirm specificity.
Researchers also utilize HRAS antibodies to explore therapeutic targeting, such as evaluating RAS pathway inhibitors or studying HRAS interactions with regulatory proteins (e.g., GAPs, GEFs). Their applications extend to drug development, biomarker discovery, and mechanistic studies of oncogenesis. High-quality HRAS antibodies are essential for advancing precision oncology and understanding RAS-driven pathologies.
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