| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RAP1GAP; KIAA0474; RAP1GA1; Rap1 GTPase-activating protein 1; Rap1GAP; Rap1GAP1 |
| Entrez GeneID | 5909 |
| WB Predicted band size | Calculated MW: 73 kDa; Observed MW: 95 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human RAP1GAP |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于RAP1GAP抗体的3篇模拟参考文献(非真实文献，仅示例)：
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1. **文献名称**: "RAP1GAP suppresses tumor progression in thyroid cancer by modulating MAPK signaling"
**作者**: Zhang L, et al.
**摘要**: 研究通过免疫组化分析发现，RAP1GAP在甲状腺癌组织中表达显著下调，其低表达与淋巴结转移和预后不良相关。体外实验表明，RAP1GAP抗体可抑制癌细胞侵袭能力，机制可能与调控Ras-MAPK通路有关。
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2. **文献名称**: "RAP1GAP as a potential biomarker for pancreatic cancer diagnosis"
**作者**: Wang Y, et al.
**摘要**: 该研究利用RAP1GAP特异性抗体检测胰腺癌患者血清样本，发现RAP1GAP蛋白水平显著高于健康对照组，提示其可能作为新型诊断标志物。进一步功能实验证实RAP1GAP通过抑制Rap1信号通路促进肿瘤增殖。
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3. **文献名称**: "Role of RAP1GAP in neuroblastoma cell differentiation and apoptosis"
**作者**: Chen H, et al.
**摘要**: 研究使用RAP1GAP抗体进行Western blot和免疫荧光分析，发现RAP1GAP在神经母细胞瘤分化过程中表达上调。敲低RAP1GAP导致细胞凋亡减少，提示其通过调控Rho家族GTP酶活性影响肿瘤细胞命运。
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(注：以上文献为模拟生成，实际研究需通过PubMed、Web of Science等平台检索真实文献。)
The RAP1GAP antibody is a crucial tool for studying the RAP1 GTPase-activating protein (RAP1GAP), a regulatory protein involved in cellular signaling pathways. RAP1GAP belongs to the GTPase-activating protein (GAP) family, which negatively regulates small GTPases like RAP1 (Ras-related protein 1) by accelerating their GTP hydrolysis, thereby switching them to an inactive GDP-bound state. RAP1 plays a central role in integrin-mediated cell adhesion, migration, proliferation, and differentiation. Dysregulation of RAP1 signaling is linked to cancer progression, metabolic disorders, and neurological diseases. RAP1GAP, through its inhibitory role, acts as a tumor suppressor in various cancers, including pancreatic, thyroid, and breast cancers, where its downregulation correlates with metastasis and poor prognosis.
The RAP1GAP antibody is widely used in research to detect RAP1GAP expression levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence. It helps elucidate RAP1GAP's functional mechanisms, such as its interaction with other signaling molecules (e.g., β-catenin, ERK) and its impact on pathways like PI3K/AKT and Wnt/β-catenin. Studies also explore its role in insulin secretion and neuronal development. Commercially available antibodies are typically validated for specificity, targeting epitopes within the conserved C-terminal catalytic domain. Understanding RAP1GAP's regulatory network via such antibodies provides insights into therapeutic strategies for diseases driven by aberrant RAP1 activity.
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