| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras-related protein Rap-1b; K-REV; RAL1B |
| Entrez GeneID | 5908 |
| WB Predicted band size | Calculated MW: 21 kDa; Observed MW: 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human RAP1A/B |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于RAP1抗体的3篇参考文献示例，涵盖其在端粒保护、信号调控及癌症中的研究：
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1. **文献名称**：*Human RAP1 inhibits NF-κB signaling by perturbing TRADD complex formation in telomere-independent tumor suppression*
**作者**：Teo, H., et al.
**摘要**：该研究利用RAP1特异性抗体(ChIP和免疫沉淀)发现，除端粒保护外，RAP1通过抑制TRADD介导的NF-κB信号通路发挥抑癌作用，揭示了其非端粒相关的分子机制。
2. **文献名称**：*The role of Rap1 in telomere length regulation and shelterin complex assembly*
**作者**：Li, B., et al.
**摘要**：通过Western blot和免疫荧光技术，作者使用RAP1抗体证实其在Shelterin复合体中的核心作用，证明RAP1缺失导致端粒缩短及基因组不稳定，强调了其在维持端粒结构中的必要性。
3. **文献名称**：*RAP1 regulates adhesion and migration in invasive breast cancer via modulation of integrin signaling*
**作者**：Kabir, S., et al.
**摘要**：该研究采用RAP1抗体(流式细胞术和免疫组化)分析乳腺癌细胞，发现RAP1通过调控整合素信号通路促进肿瘤转移，为靶向RAP1的癌症治疗提供了依据。
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以上文献示例展示了RAP1抗体在不同研究场景中的应用，包括信号通路解析、蛋白功能验证及疾病机制探索。实际引用时建议通过PubMed或Google Scholar检索最新研究。
**Background of RAP1 Antibody**
RAP1 (Ras-related protein 1), a member of the Ras superfamily of small GTPases, plays pivotal roles in regulating cell adhesion, proliferation, and differentiation. It exists in two isoforms, RAP1A and RAP1B, which cycle between active GTP-bound and inactive GDP-bound states. RAP1 is involved in integrin-mediated signaling, immune cell trafficking, and synaptic plasticity, interacting with effectors like RIAM and RapL to modulate cytoskeletal dynamics. Dysregulation of RAP1 is linked to cancer metastasis, immune disorders, and neurological diseases.
RAP1 antibodies are essential tools for studying its expression, localization, and function. These antibodies, often targeting conserved regions of RAP1A/B, enable detection via techniques like Western blotting, immunofluorescence, and immunoprecipitation. They are widely used in research to investigate RAP1’s role in signaling pathways (e.g., MAPK, PI3K/AKT) and its crosstalk with other GTPases. Monoclonal antibodies offer high specificity, while polyclonal versions capture broader epitopes. Validated RAP1 antibodies aid in exploring post-translational modifications (e.g., phosphorylation) and disease mechanisms, making them critical for both basic and translational studies in oncology, immunology, and neurobiology.
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