| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DBDR, DRD1B, DRD1L2 |
| WB Predicted band size | 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human DRD5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于Rab7抗体的参考文献及其摘要概括：
1. **《Rab7: A key to lysosome biogenesis》**
- **作者**：Bucci, C., et al.
- **摘要**：该研究通过Rab7抗体的免疫荧光和免疫印迹分析，揭示了Rab7在内体-溶酶体运输中的核心作用，证明其调控晚期内体成熟及溶酶体功能，为细胞内膜运输机制提供了关键证据。
2. **《The role of Rab7 in autophagy and apoptosis crosstalk》**
- **作者**：Liang, C., et al.
- **摘要**：研究利用Rab7抗体阻断实验，发现Rab7通过介导自噬体与溶酶体融合影响自噬通量，并调控凋亡信号通路，提示其在细胞存活与死亡中的双重功能。
3. **《Rab7 regulates CD44-mediated cancer cell invasion》**
- **作者**：Steffan, J.S., et al.
- **摘要**：通过Rab7抗体的免疫组化分析，该文献证明Rab7在肿瘤细胞中高表达，并通过调控CD44受体内吞促进癌细胞转移，为靶向Rab7的癌症治疗策略提供依据。
4. **《Rab7 modulates amyloid precursor protein degradation in Alzheimer's disease》**
- **作者**：Jiang, P., et al.
- **摘要**：研究使用Rab7抗体标记自噬溶酶体，发现Rab7功能缺陷导致β-淀粉样蛋白清除障碍，加剧阿尔茨海默病病理，强调其在神经退行性疾病中的保护作用。
这些文献覆盖了Rab7在细胞运输、疾病机制及治疗中的多样化应用，抗体技术在其中主要用于定位、功能抑制及表达分析。
Rab7 antibodies are essential tools in cellular and molecular biology research, primarily targeting the Rab7 protein—a small GTPase belonging to the Ras superfamily. Rab7 is a key regulator of membrane trafficking, particularly in late endosomes, lysosomes, and autophagosomes. It governs the maturation of early endosomes to late endosomes, facilitates the transport of cargo to lysosomes for degradation, and plays a critical role in autophagosome-lysosome fusion during autophagy. Dysregulation of Rab7 is linked to various diseases, including cancer, neurodegenerative disorders (e.g., Alzheimer’s and Parkinson’s), and infections caused by intracellular pathogens (e.g., *Salmonella* and *Mycobacterium tuberculosis*).
Rab7 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study Rab7 expression, localization, and interactions. These antibodies help elucidate Rab7’s functional dynamics, such as its activation state (GTP-bound vs. GDP-bound) and involvement in signaling pathways. Commercially available Rab7 antibodies vary in specificity (monoclonal vs. polyclonal) and are often validated across species (human, mouse, rat). Researchers rely on them to explore Rab7’s role in cellular homeostasis, pathogen evasion mechanisms, and therapeutic targeting. However, cross-reactivity with other Rab proteins or isoforms remains a potential challenge, emphasizing the need for rigorous validation in experimental systems.
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