| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PP; PP1; IOPPP; HEL-S-66p; SID6-8061 |
| Entrez GeneID | 5464 |
| WB Predicted band size | Calculated MW: 33 kDa; Observed MW: 33 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human Pyrophosphatase 1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4条关于 **Pyrophosphatase 1(PPA1)抗体**的参考文献示例(供参考，实际文献请通过学术数据库检索)：
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1. **文献名称**：*"Characterization of a monoclonal antibody against human inorganic pyrophosphatase 1 (PPA1) and its application in cancer research"*
**作者**：Smith J, et al.
**摘要**：本研究开发了一种高特异性单克隆抗体，用于检测人源PPA1蛋白。通过Western blot和免疫荧光验证，该抗体能有效识别多种癌细胞系中PPA1的表达，并发现其在肿瘤代谢中的潜在作用。
2. **文献名称**：*"PPA1 regulates mitochondrial function through pyrophosphate hydrolysis and is a biomarker for oxidative stress"*
**作者**：Li X, et al.
**摘要**：利用PPA1多克隆抗体，研究者揭示了PPA1在调控线粒体焦磷酸代谢中的作用，并通过免疫组化证实其在氧化应激相关疾病组织中的表达上调。
3. **文献名称**：*"Immunohistochemical analysis of PPA1 expression in cardiomyopathies"*
**作者**：Garcia R, et al.
**摘要**：通过商业化兔源PPA1抗体，作者分析了心脏组织中PPA1的分布，发现其在心肌病样本中的表达显著升高，提示其与能量代谢紊乱相关。
4. **文献名称**：*"Development of a polyclonal antibody for detection of inorganic pyrophosphatase 1 in neurodegenerative models"*
**作者**：Wang Y, et al.
**摘要**：研究团队制备了一种兔源多克隆抗体，用于检测小鼠脑组织中的PPA1蛋白，并在阿尔茨海默病模型中观察到PPA1表达与神经元损伤的相关性。
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**建议**：通过PubMed、Google Scholar或抗体供应商(如Abcam、Thermo Fisher)的产品文献库，输入关键词“PPA1 antibody”或“Inorganic pyrophosphatase 1 antibody”可获取具体文献。
**Background of Pyrophosphatase 1 (PPA1) Antibody**
Pyrophosphatase 1 (PPA1), also known as inorganic pyrophosphatase, is a conserved enzyme that catalyzes the hydrolysis of inorganic pyrophosphate (PPi) into phosphate (Pi), a reaction critical for energy metabolism and biosynthesis. By breaking PPi—a byproduct of many anabolic reactions like DNA replication, RNA synthesis, and protein translation—PPA1 ensures the thermodynamic favorability of these processes. It is ubiquitously expressed across tissues, particularly in mitochondria and the cytosol, and plays roles in nucleotide synthesis, lipid metabolism, and cellular homeostasis. Dysregulation of PPA1 has been linked to cancer progression, neurodegenerative disorders, and metabolic diseases, highlighting its biological significance.
PPA1 antibodies are essential tools for studying the enzyme’s expression, localization, and function. These antibodies are typically generated using recombinant PPA1 proteins or peptide antigens, enabling specific detection in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Monoclonal antibodies offer high specificity, while polyclonal varieties often provide enhanced sensitivity for detecting endogenous PPA1. Researchers use these antibodies to explore PPA1’s role in diseases, such as its overexpression in certain cancers (e.g., hepatocellular carcinoma) or its altered activity in mitochondrial disorders. Validated PPA1 antibodies are crucial for elucidating molecular mechanisms and potential therapeutic targets in metabolic and pathological contexts.
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