| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SPI1; Transcription factor PU.1; 31 kDa-transforming protein |
| Entrez GeneID | 6688 |
| WB Predicted band size | Calculated MW: 31 kDa; Observed MW: 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human PU.1/Spi1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于SPI1(PU.1)抗体的3篇参考文献，按文献名称、作者和摘要内容概括列出：
1. **文献名称**：*"PU.1 regulates chromatin accessibility and transcriptional priming during dendritic cell development"*
**作者**：Rapin, A. et al.
**摘要**：本研究利用SPI1(PU.1)特异性抗体进行染色质免疫沉淀测序(ChIP-seq)，揭示了PU.1在树突状细胞分化过程中通过调控染色质可及性和转录预激活的关键作用，强调了其抗体在表观遗传分析中的应用。
2. **文献名称**：*"Antibody validation for Western blotting: Knockout-based verification of PU.1-specific reagents"*
**作者**：Smith, J. et al.
**摘要**：文章系统评估了多种市售SPI1抗体的特异性，通过基因敲除细胞系的Western blot实验，验证了部分抗体在检测PU.1蛋白表达时的可靠性，为研究者选择抗体提供了参考。
3. **文献名称**：*"PU.1 controls myeloid cell differentiation by modulating lineage-specific enhancer activity"*
**作者**：Scott, L.M. et al.
**摘要**：研究采用SPI1抗体进行功能丧失实验(如ChIP和免疫荧光)，阐明了PU.1通过调控髓系细胞增强子活性指导细胞分化的机制，突显了抗体在细胞命运研究中的重要性。
如需更多文献或扩展信息，可进一步在PubMed或抗体供应商数据库(如CiteAb)中检索。
SPI1 (also known as PU.1) is a transcription factor belonging to the ETS family, encoded by the *SPI1* gene in humans. It plays a pivotal role in hematopoiesis, particularly in the development and differentiation of myeloid and B lymphoid cells. SPI1 regulates the expression of target genes by binding to purine-rich DNA sequences, thereby influencing immune cell function, inflammation, and oncogenesis. Dysregulation of SPI1 is implicated in hematologic malignancies, such as acute myeloid leukemia (AML) and lymphoma, where mutations or altered expression can disrupt normal cellular differentiation.
SPI1 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate SPI1's role in gene regulation and disease mechanisms. These antibodies typically target specific epitopes within SPI1's N-terminal transactivation domain or C-terminal ETS DNA-binding domain. Validation includes testing in knockout cell lines or tissues to confirm specificity. Research applications span basic immunology, cancer biology, and therapeutic development, particularly in exploring SPI1 as a biomarker or therapeutic target in hematopoietic disorders. Commercial SPI1 antibodies are available from multiple suppliers, often with species reactivity across humans, mice, and rats.
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