| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PRP4; RP70; HPRP4; Prp4p; HPRP4P; SNRNP60 |
| Entrez GeneID | 9128 |
| WB Predicted band size | Calculated MW: 58 kDa; Observed MW: 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human PRPF4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于PRPF4抗体的3篇参考文献(均为虚拟示例，实际文献需通过学术数据库检索)：
1. **标题**: "PRPF4 regulates spliceosome assembly through direct interaction with U5 snRNP components"
**作者**: Zhang L, et al.
**摘要**: 本研究利用PRPF4抗体进行免疫共沉淀实验，发现PRPF4通过结合U5 snRNP蛋白调控剪接体早期组装过程，揭示了其在pre-mRNA剪接中的关键作用。
2. **标题**: "Aberrant expression of PRPF4 in hepatocellular carcinoma correlates with poor prognosis"
**作者**: Wang Y, et al.
**摘要**: 通过Western blot和免疫组化(使用PRPF4抗体)，作者发现PRPF4在肝癌组织中高表达，其水平与患者生存率负相关，提示其作为潜在肿瘤标志物的可能性。
3. **标题**: "A novel PRPF4 mutation causes autosomal dominant retinitis pigmentosa via splicing defects"
**作者**: Li X, et al.
**摘要**: 该研究在视网膜色素变性家系中发现PRPF4基因突变，通过免疫荧光(使用PRPF4特异性抗体)证实突变导致蛋白亚细胞定位异常，进而引发光感受器细胞凋亡。
注：以上为示例性内容，实际文献请通过PubMed、Google Scholar等平台以"PRPF4 antibody"或"PRPF4 function"为关键词检索，重点关注涉及抗体应用(如Western blot、IP、IF等实验方法)的功能研究或疾病机制论文。
The PRPF4 antibody targets the pre-mRNA processing factor 4 (PRPF4), a component of the spliceosome complex responsible for catalyzing precursor mRNA splicing in eukaryotic cells. PRPF4. also known as PRP4 kinase, is a serine/threonine kinase that plays a critical role in spliceosome assembly and regulation by phosphorylating spliceosomal proteins during dynamic remodeling. It is constitutively expressed in most tissues and localized predominantly in the nucleus.
PRPF4 antibodies are widely used in research to investigate spliceosome dynamics, RNA processing mechanisms, and their links to diseases. Studies employ these antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect PRPF4 expression levels, subcellular localization, and interactions with other splicing factors. Dysregulation of PRPF4 has been implicated in pathologies such as cancer and neurodegenerative disorders. For example, aberrant PRPF4 expression or mutations may disrupt alternative splicing patterns, contributing to oncogenesis or neuronal dysfunction.
Additionally, PRPF4 antibodies aid in exploring its non-canonical roles, including cell cycle regulation and DNA damage response. Their utility extends to screening therapeutic targets and biomarkers in diseases linked to splicing defects. As splicing errors underlie numerous genetic disorders, PRPF4 remains a focal point in understanding molecular pathogenesis and developing precision therapies.
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