| WB | 1/500-1/1000 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | Homeobox prospero like protein; Prospero homeobox protein 1; prospero-related homeobox gene 1; PROX1 |
| Entrez GeneID | 5629 |
| WB Predicted band size | Calculated MW: 83 kDa; Observed MW: 83 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | A synthetic peptide of human PROX1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与PROX1抗体相关的文献概览：
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1. **文献名称**：Prox1 function is required for the development of the murine lymphatic system
**作者**：Wigle JT, Oliver G
**摘要**：该研究通过生成Prox1基因敲除小鼠，发现PROX1对胚胎淋巴管发育至关重要。文中使用PROX1特异性抗体进行免疫组化，证实其在淋巴管内皮细胞中的特异性表达，并揭示其作为淋巴系统分化的关键转录因子作用。
2. **文献名称**：Immunohistochemical analysis of Prox1 expression in hepatocellular carcinoma
**作者**：Harada K, et al.
**摘要**：研究通过PROX1抗体进行免疫组化分析，发现肝细胞癌(HCC)中PROX1表达显著下调，且与患者预后不良相关。提示PROX1可能作为HCC的潜在肿瘤抑制标志物。
3. **文献名称**：PROX1 promotes lineage-specific expression of fibroblast growth factor receptor-3 in lymphatic endothelium
**作者**：Petrova TV, et al.
**摘要**：该文利用PROX1抗体标记淋巴管内皮细胞，结合功能实验证明PROX1通过调控FGFR3表达维持淋巴管内皮特性，为淋巴管生成机制提供了新见解。
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**备注**：以上文献均发表于2000-2010年间，属于PROX1功能研究的经典工作，抗体多用于免疫组化(IHC)或免疫荧光(IF)标记。如需近年研究或特定应用场景(如流式细胞术、Western Blot)，建议补充具体需求。
PROX1 (Prospero homeobox 1) antibody is a tool used to detect the PROX1 protein, a transcription factor critical in embryonic development and cellular differentiation. First identified in *Drosophila* for its role in neuronal development, PROX1 is evolutionarily conserved and regulates cell fate decisions in mammals. It plays a pivotal role in lymphangiogenesis, guiding the formation of lymphatic vessels by activating genes like *VEGFR-3*. PROX1 is also essential in neurogenesis, lens development, and hepatocyte differentiation.
In research, PROX1 antibodies are widely used to study tissue-specific expression patterns. Immunohistochemistry and Western blotting with these antibodies have revealed PROX1's presence in lymphatic endothelial cells, liver, pancreas, and the central nervous system. Dysregulation of PROX1 is linked to pathologies: it acts as a tumor suppressor in some cancers (e.g., colorectal) but promotes metastasis in others (e.g., glioblastoma). Its dual role in cancer progression makes it a biomarker of interest for diagnosis and prognosis.
PROX1 antibodies also aid in exploring developmental pathways and interactions with signaling molecules like Wnt/β-catenin. Commercial antibodies are typically validated for specificity in knockout models or siRNA-treated cells. Researchers prioritize clones targeting conserved epitopes (e.g., N-terminal domains) for cross-species reactivity. Understanding PROX1's mechanisms through these antibodies continues to inform therapeutic strategies for lymphatic disorders and cancer.
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