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产品描述：人髓鞘碱性蛋白(MBP)抗体酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、脑脊液（CSF）样本中的MBP Ab含量。人髓鞘碱性蛋白（MBP）抗体是一种针对髓鞘碱性蛋白的自身免疫抗体，与多发性硬化症（MS）等脱髓鞘疾病的发生密切相关。研究显示，MBP抗体在神经组织细胞膜上裂解MBP，导致髓鞘损伤，进而引发MS。目前，针对MBP抗体的研究主要集中在揭示其与MS等疾病的关系，以及开发针对该靶点的治疗策略。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研领域中对神经系统疾病模型的机制研究、药物干预效果评估或生物标志物探索，例如多发性硬化症、脑创伤或神经退行性疾病的体外实验分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MBP Ab
种属：Homo sapiens (Human)
样本类型：serum, cerebrospinal fluid (CSF)
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MBP antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 85-103 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 84-100 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 82-106 | |||||
回收率：
| The recovery of human MBP antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 83-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.5952.6582.6272.524 202.1212.1362.1292.026 101.4671.4731.4701.367 50.8480.8960.8720.769 2.50.4750.4940.4850.382 1.250.3110.2980.3050.202 0.6250.2340.2530.2440.141 00.1020.1040.103 | ||||||
货期：3-5 working days
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