关键信息
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基因名
Norrin
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简介
Norrin protein activates canonical Wnt signaling by binding to FZD4 and LRP5, promotes retinal vascularization and stabilizes β-catenin (CTNNB1). It cooperates with TSPAN12 to independently activate FZD4, suggesting a Wnt-independent mechanism. Norrin Protein, Human is the recombinant human-derived Norrin protein, expressed by E. coli , with tag free.
- 应用
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生物活性
Measured by its binding ability in a functional ELISA. When Human Norrin is coated at 0.5 μg/mL (100 μL/well) can bind Human Frizzled4. The ED50 for this effect is 86.34 ng/mL. Measured by its binding ability in a functional ELISA. When Human Norrin is coated at 0.5 μg/mL (100 μL/well) can bind Human Frizzled4. The ED50 for this effect is 86.34 ng/mL.
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别名
Norrin; NDP; Norrie disease protein; X-linked exudative vitreoretinopathy 2 protein; EVR2
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种属
Human
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q00604
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表达区间
K25-S133
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蛋白长度
Full Length of Mature Protein
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分子量
13 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Norrin is a secreted cysteine-rich growth factor that primarily plays a pivotal role in the development and maintenance of the retinal vasculature and the inner ear. It is encoded by the NDP gene, and mutations in this gene are implicated in Norrie disease, a rare genetic disorder leading to vision and hearing loss. Understanding the biological functions and mechanisms of Norrin is crucial for elucidating its role in angiogenesis and neural development. Recent studies have highlighted its interactions with Frizzled-4 and β-catenin in the Wnt signaling pathway, which are essential for endothelial cell communication and blood-brain barrier integrity. Given its significance in both normal physiological processes and disease states, characterizing Norrin as a recombinant protein is essential for exploring therapeutic applications. Researchers have been focusing on producing recombinant Norrin to investigate its structural properties, biological activities, and potential as a therapeutic agent for ocular and auditory disorders. By utilizing advanced protein engineering techniques, scientists aim to enhance the understanding of Norrin's functional mechanisms and develop targeted interventions that could mitigate the effects of Norrie disease and similar conditions. As studies progress, the promise of recombinant Norrin in regenerative medicine and its implications for treating vascular and sensory disorders continue to garner attention in the biomedical research community.
