| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PGES; MPGES; PIG12; PP102; PP1294; MGST-IV; MGST1L1; TP53I12; mPGES-1; MGST1-L1 |
| Entrez GeneID | 9536 |
| WB Predicted band size | Calculated MW: 17 kDa; Observed MW: 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Prostaglandin E Synthase |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Prostaglandin E Synthase(PGES)抗体的参考文献及简要摘要：
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1. **"Molecular identification of cytosolic prostaglandin E synthase that is functionally coupled with cyclooxygenase-1 in immediate prostaglandin E2 biosynthesis"**
*Authors: F. Kojima, H. Naraba, S. Kawai*
**摘要**：该研究通过免疫印迹和免疫组织化学方法，使用特异性抗体验证了胞质型前列腺素E合成酶(cPGES)与COX-1的共定位，揭示了其在炎症反应中快速生成PGE₂的作用。
2. **"Regulation of prostaglandin E2 biosynthesis by inducible membrane-associated prostaglandin E synthase that acts in concert with cyclooxygenase-2"**
*Authors: M. Murakami, I. Kudo*
**摘要**：文章利用针对膜相关型PGES(mPGES-1)的特异性抗体，证实其在COX-2依赖的PGE₂合成中的关键作用，并探讨了抗体在炎症和癌症模型中的应用。
3. **"Purification and characterization of membrane-bound prostaglandin E synthase from bovine heart"**
*Authors: T. Tanioka, Y. Sugimoto, A. Ichikawa*
**摘要**：研究通过开发兔源多克隆抗体，成功纯化并鉴定了心肌组织中的mPGES-1.抗体特异性验证显示其适用于酶活性抑制和蛋白定位分析。
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**备注**：上述文献均涉及PGES抗体的开发、验证或应用，涵盖炎症、癌症等研究场景。如需具体抗体货号或实验细节，建议补充目标亚型(如mPGES-1/cPGES)。
Prostaglandin E synthase (PGES) is a key enzyme in the biosynthesis of prostaglandin E₂ (PGE₂), a lipid mediator involved in inflammation, pain, fever, and cellular homeostasis. Three isoforms of PGES have been identified: microsomal PGES-1 (mPGES-1), mPGES-2. and cytosolic PGES (cPGES). Among these, mPGES-1 is the most studied due to its inducible expression under inflammatory conditions and its role as a downstream target of cyclooxygenase-2 (COX-2) in the arachidonic acid pathway. Antibodies targeting PGES isoforms are critical tools for investigating their expression, localization, and functional roles in physiological and pathological processes.
PGES antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein levels in tissues or cultured cells. Specificity is crucial, as mPGES-1. mPGES-2. and cPGES differ in structure, subcellular localization, and regulatory mechanisms. For example, mPGES-1 antibodies help study its upregulation in inflammatory diseases (e.g., arthritis) and cancers, while cPGES antibodies are employed to explore constitutive PGE₂ production in homeostatic processes. Researchers also use these antibodies to evaluate the efficacy of therapeutic agents targeting PGES isoforms, particularly mPGES-1 inhibitors, which are investigated as alternatives to NSAIDs with fewer gastrointestinal side effects. Validation of PGES antibodies includes testing in knockout models or siRNA-treated cells to confirm target specificity, ensuring reliable data in studies linking PGES activity to disease mechanisms or drug development.
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