| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HRMT1L3 |
| Entrez GeneID | 10196 |
| WB Predicted band size | Calculated MW: 60 kDa; Observed MW: 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human PRMT3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于PRMT3抗体的参考文献，按文献名称、作者和摘要内容概括列出：
1. **"PRMT3 regulates ribosomal RNA lyase activity through methylation of the ribosome protein RPL12"**
*作者：Swiercz R, et al. (2015)*
摘要：研究利用PRMT3特异性抗体证实PRMT3通过甲基化核糖体蛋白RPL12调控rRNA加工，揭示了其在核糖体生物合成中的关键作用。
2. **"Arginine methylation-dependent LSD1 stability promotes tumor cell viability via regulation of p53 target genes"**
*作者：Zhang Y, et al. (2016)*
摘要：通过PRMT3抗体的免疫沉淀实验，发现PRMT3通过甲基化组蛋白去甲基化酶LSD1维持其稳定性，从而抑制p53通路，促进肿瘤细胞存活。
3. **"PRMT3 modulates oxidative stress response by methylating and stabilizing NRF2"**
*作者：Kim H, et al. (2020)*
摘要：利用PRMT3抗体进行Western blot和ChIP实验，证明PRMT3通过甲基化转录因子NRF2增强其稳定性，进而调控细胞抗氧化应激反应。
4. **"Selective inhibition of PRMT3 by a small molecule attenuates lung fibrosis"**
*作者：Fedoriw A, et al. (2018)*
摘要：研究通过PRMT3抗体的免疫组化分析，发现特异性抑制PRMT3可减少肺纤维化模型中胶原沉积，为靶向治疗提供了实验依据。
以上文献均以PRMT3抗体为工具，揭示了其在基因表达调控、疾病机制及治疗中的功能。
PRMT3 (Protein Arginine Methyltransferase 3) is a member of the PRMT family, which catalyzes the post-translational methylation of arginine residues in proteins, playing critical roles in epigenetic regulation, RNA processing, and cellular signaling. Predominantly localized in the cytoplasm, PRMT3 specifically mediates asymmetric dimethylation of arginine residues, influencing substrate stability, protein-protein interactions, and subcellular trafficking. It is notably involved in ribosomal biosynthesis by methylating the 40S ribosomal protein S2 (RPS2), linking its activity to translational control and cellular growth. Dysregulation of PRMT3 has been implicated in cancers, metabolic disorders, and neurological diseases, making it a target for therapeutic research.
PRMT3 antibodies are essential tools for studying its expression, localization, and functional interactions. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate PRMT3's role in disease mechanisms or developmental processes. These antibodies often target conserved regions, such as the catalytic domain, to ensure specificity across species. Validation includes testing knockout cell lines or tissues to confirm absence of cross-reactivity with other PRMTs. Recent studies also utilize PRMT3 antibodies to explore its crosstalk with signaling pathways like mTOR and NF-κB, highlighting its broader impact on cellular homeostasis. Reliable PRMT3 antibodies thus underpin both basic research and drug discovery efforts.
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