| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PPP1CB; Serine/threonine-protein phosphatase PP1-beta catalytic subunit; PP-1B; PPP1CD |
| Entrez GeneID | 5500 |
| WB Predicted band size | Calculated MW: 37 kDa; Observed MW: 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human PPP1CB |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及PP1Cβ(PPP1CB)抗体的参考文献摘要(虚构示例，仅供参考格式)：
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1. **文献名称**: *Characterization of PP1Cβ-specific antibodies for studying neuronal phosphatase signaling*
**作者**: Smith A et al.
**摘要**: 本研究开发了一种高特异性兔源多克隆抗体，靶向PP1Cβ(PPP1CB)的C端表位。通过Western blot验证其在脑组织裂解液中的特异性，并利用免疫荧光技术揭示PP1Cβ在小鼠海马神经元突触后区域的富集分布，支持其在突触可塑性中的潜在作用。
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2. **文献名称**: *PP1Cβ isoform-selective regulation of glucose metabolism in hepatocytes*
**作者**: Chen L et al.
**摘要**: 通过商业化小鼠单克隆PP1Cβ抗体(克隆号：3D8)，研究团队发现PP1Cβ在肝细胞胰岛素信号通路中通过去磷酸化调控GSK3β活性，影响糖原合成。抗体特异性经siRNA敲低实验验证，证实其适用于代谢相关蛋白互作研究。
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3. **文献名称**: *Development of a phospho-specific antibody for PP1Cβ Thr320 phosphorylation*
**作者**: Kimura T et al.
**摘要**: 该文献报道了一种新型磷酸化抗体，靶向PP1Cβ Thr320位点(抑制性磷酸化位点)。作者在心肌缺血模型中利用该抗体揭示PP1Cβ活性受氧化应激动态调控的机制，为心血管疾病治疗提供新靶点。
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注：以上内容为模拟生成，实际文献需通过PubMed或Google Scholar检索关键词(如“PP1Cβ antibody”“PPP1CB antibody”)获取。
The PP1C beta antibody is a crucial tool for investigating the function and regulation of protein phosphatase 1 catalytic subunit beta (PP1Cβ), a member of the serine/threonine phosphatase family. PP1Cβ, encoded by the PPP1CB gene, forms the catalytic core of protein phosphatase 1 (PP1), which regulates diverse cellular processes, including cell cycle progression, metabolism, and signal transduction. PP1 achieves functional specificity by interacting with regulatory subunits that determine substrate targeting and subcellular localization. The beta isoform (PP1Cβ) is ubiquitously expressed and plays roles in neuronal signaling, muscle contraction, and cancer pathways.
PP1C beta antibodies are typically generated using immunogenic peptides or recombinant proteins corresponding to conserved or isoform-specific regions. These antibodies enable the detection of endogenous PP1Cβ in applications like Western blotting, immunofluorescence, and immunoprecipitation. Specificity is critical, as PP1C isoforms (α, β, γ) share high sequence homology. Validated antibodies help study PP1Cβ expression patterns, post-translational modifications (e.g., phosphorylation), and interactions with regulatory proteins.
Research using PP1C beta antibodies has elucidated its involvement in diseases, including Alzheimer’s (via tau dephosphorylation) and cancer (through cell cycle dysregulation). These reagents are essential for dissecting PP1’s isoform-specific roles in cellular homeostasis and pathology.
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