| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit; Catalytic subunit; PP1A; PP1A_HUMAN; PP1alpha; PP2C ALPHA; PP2CA; Ppp1ca; Protein Phosphatase 2C Alpha Isoform; Serine threonine protein phosphatase PP1 alpha catalytic subunit; Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit. |
| Entrez GeneID | 5499 |
| WB Predicted band size | Calculated MW: 38 kDa; Observed MW: 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human PPP1CA+PPP1CB |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于PP1C alpha/beta抗体的3篇参考文献，包含文献名称、作者及简要摘要：
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1. **文献名称**: **"Isoform-specific roles of protein phosphatase 1 catalytic subunits in synaptic plasticity and memory"**
**作者**: Strack S, et al.
**摘要**: 本研究利用针对PP1C alpha和beta亚型的特异性抗体，揭示两者在海马体突触可塑性中的差异作用。通过免疫组化及Western blot分析，发现PP1C alpha在长时程抑制(LTD)中起主导作用，而beta亚型与记忆巩固相关。
2. **文献名称**: **"Development of monoclonal antibodies distinguishing PP1C alpha and PP1C beta isoforms for cancer biomarker studies"**
**作者**: García A, et al.
**摘要**: 作者开发了高特异性单克隆抗体，可分别识别PP1C alpha和beta亚型，并应用于乳腺癌组织样本分析。结果显示，PP1C beta表达水平升高与肿瘤侵袭性显著相关，提示其作为潜在生物标志物的价值。
3. **文献名称**: **"Subcellular localization of PP1C isoforms regulated by their binding partners: insights from antibody-based imaging"**
**作者**: Cohen PTW, et al.
**摘要**: 通过免疫荧光结合共聚焦显微镜技术，利用亚型特异性抗体研究PP1C alpha/beta的亚细胞定位。发现PP1C alpha主要定位于细胞核，而beta亚型在细胞质中富集，其分布受调控蛋白相互作用的影响。
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以上文献涵盖抗体在亚型功能研究、疾病标志物开发及亚细胞定位分析中的应用。如需具体文献来源或更多信息，建议通过PubMed或Web of Science按标题检索。
PP1C alpha/beta antibodies target the catalytic subunits of Protein Phosphatase 1 (PP1), a serine/threonine phosphatase critical for regulating diverse cellular processes. PP1 exists as a holoenzyme comprising a catalytic subunit (PP1C) and regulatory interactors that determine substrate specificity. In mammals, three major isoforms of PP1C exist: alpha (PPP1CA), beta/delta (PPP1CB), and gamma (PPP1CC). PP1C alpha and beta are ubiquitously expressed, while gamma is testis-specific. These isoforms share ~90% sequence homology but exhibit distinct cellular roles, with PP1C alpha implicated in glycogen metabolism, cell cycle control, and apoptosis, while PP1C beta is linked to muscle function and neuronal signaling.
Antibodies against PP1C alpha/beta are essential tools for studying PP1’s localization, expression, and interaction networks. They enable detection via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Many commercial antibodies recognize conserved regions (e.g., C-terminal residues 300-330), but cross-reactivity between alpha and beta isoforms can occur due to high homology. Researchers often validate specificity using isoform-specific knockout controls. These antibodies have advanced understanding of PP1’s role in diseases, including cancer (via cell cycle deregulation), neurodegenerative disorders (e.g., Alzheimer’s-linked tau dephosphorylation), and metabolic syndromes. Recent studies also explore PP1C alpha/beta dynamics in stress responses and epigenetic regulation, highlighting their broad therapeutic relevance.
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