| WB | 1/500-1/1000 | Human,Mouse,Rat,Hamster |
| IF | 1/20 | Human,Mouse,Rat,Hamster |
| IHC | 1/50-1/100 | Human,Mouse,Rat,Hamster |
| ICC | 1/50-1/200 | Human,Mouse,Rat,Hamster |
| FCM | 咨询技术 | Human,Mouse,Rat,Hamster |
| Elisa | 咨询技术 | Human,Mouse,Rat,Hamster |
| Aliases | PPP2CA; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C |
| Entrez GeneID | 5515 |
| WB Predicted band size | Calculated MW: 36 kDa; Observed MW: 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Hamster |
| Immunogen | A synthetic peptide of human PP2A alpha |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于PP2A α/β抗体的3篇参考文献示例(注：文献为假设示例，实际引用需核实)：
1. **文献名称**：*"Differential roles of PP2A catalytic subunits α and β in neuronal development"*
**作者**：Johnson R. et al.
**摘要**：通过特异性PP2A α和β抗体，研究两者在小鼠神经元发育中的表达差异，发现PP2A β在轴突导向中起关键作用，Western blot和免疫荧光验证抗体特异性。
2. **文献名称**：*"PP2A subunit-specific antibodies reveal β isoform dysregulation in breast cancer"*
**作者**：Martinez L. et al.
**摘要**：利用商业化PP2A α/β抗体分析乳腺癌组织，发现PP2A β表达降低与患者预后不良相关，并通过siRNA敲除实验证实抗体可靠性。
3. **文献名称**：*"Validation of PP2A catalytic subunit antibodies for phosphorylation studies"*
**作者**：Kim S. et al.
**摘要**：系统评估多种PP2A α/β抗体的交叉反应性，推荐特定克隆号(如β亚基的E155)用于免疫沉淀和磷酸化蛋白检测，适用于心脏疾病模型研究。
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**提示**：实际研究中建议通过PubMed或Web of Science检索最新文献，使用关键词“PP2A catalytic subunit antibody validation”或结合具体应用(如“PP2A alpha/beta antibody Alzheimer’s”)筛选高引论文。部分常用抗体供应商(如Cell Signaling Technology)的技术手册也可能提供关键参考文献。
Protein phosphatase 2A (PP2A) is a highly conserved serine/threonine phosphatase critical for regulating diverse cellular processes, including cell cycle progression, apoptosis, and signal transduction. The PP2A holoenzyme typically consists of a structural scaffold subunit (A), a catalytic subunit (C), and a regulatory subunit (B), which determine substrate specificity and localization. The A subunit exists as two paralogs, PP2A-alpha (PR65α) and PP2A-beta (PR65β), encoded by the PPP2R1A and PPP2R1B genes, respectively. These isoforms share ~87% amino acid sequence identity but exhibit tissue-specific expression and functional divergence. PP2A-alpha is ubiquitously expressed, while PP2A-beta shows more restricted expression, particularly in neuronal and endocrine tissues.
Antibodies targeting PP2A-alpha/beta are essential tools for studying PP2A complex composition, localization, and function. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to investigate PP2A's roles in diseases like cancer, neurodegenerative disorders, and cardiovascular conditions. Dysregulation of PP2A subunits has been linked to tumor suppression (e.g., PPP2R1A mutations in endometrial and lung cancers) and tau hyperphosphorylation in Alzheimer’s disease. Specific alpha/beta antibodies help distinguish isoform-specific PP2A functions, though cross-reactivity remains a consideration due to their high homology. These antibodies have become pivotal in both basic research and therapeutic development, particularly in studies exploring PP2A reactivation as a cancer treatment strategy.
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