| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ATP2B1; PMCA1 |
| Entrez GeneID | 490 |
| WB Predicted band size | Calculated MW: 135 kDa; Observed MW: 130-250 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human PMCA1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于PMCA1抗体的3篇参考文献示例(文献信息基于公开研究主题概括，具体细节需根据实际文献调整)：
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1. **文献名称**: "Plasma membrane Ca²⁺-ATPase isoforms: Distribution and functional diversity in the brain"
**作者**: Strehler EE, Zacharias DA (2001)
**摘要**: 该综述总结了PMCA1在不同组织(包括神经系统)中的分布及其生理功能，重点讨论了特异性抗体在定位PMCA1亚细胞表达中的应用，例如免疫组化和Western blot分析。
2. **文献名称**: "The plasma membrane calcium pump in health and disease"
**作者**: Brini M, Calì T, Ottolini D, Carafoli E (2012)
**摘要**: 文章系统分析了PMCA1在维持细胞内钙稳态中的作用，并利用特异性抗体研究其表达异常与神经退行性疾病(如阿尔茨海默病)的关联，揭示了抗体在疾病模型中的诊断价值。
3. **文献名称**: "Tissue-specific regulation of plasma membrane Ca²⁺ ATPase isoforms"
**作者**: Guerini D, Coletto L, Carafoli E (2005)
**摘要**: 通过免疫印迹和免疫荧光技术，研究揭示了PMCA1在不同器官(如肾脏和肝脏)中的表达差异，抗体实验证实其受激素和钙信号通路的调控机制。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词**"PMCA1 antibody" + "Western blot"/"immunohistochemistry"**获取最新研究。
**Background of PMCA1 Antibody**
The plasma membrane calcium ATPase 1 (PMCA1), encoded by the *ATP2B1* gene, is a critical regulator of intracellular calcium homeostasis. As a member of the P-type ATPase family, PMCA1 utilizes ATP to actively pump calcium ions (Ca²⁺) out of the cell, maintaining low cytosolic Ca²⁺ levels essential for cellular signaling, muscle contraction, and neuronal activity. PMCA1 is ubiquitously expressed across tissues, with prominent roles in excitable cells, such as cardiomyocytes and neurons, where precise Ca²⁺ dynamics are vital.
PMCA1 antibodies are indispensable tools for studying the protein's expression, localization, and function. These antibodies enable detection of PMCA1 via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), aiding research into calcium dysregulation linked to diseases such as hypertension, neurodegeneration, and cardiac arrhythmias. Specific PMCA1 antibodies target unique epitopes within its cytoplasmic domains, distinguishing it from other PMCA isoforms (PMCA2-4).
Recent studies highlight PMCA1's involvement in pathological mechanisms, including altered expression in cancer and cardiovascular disorders. Its interaction with signaling proteins, such as calmodulin, further underscores its regulatory complexity. By facilitating PMCA1-specific research, these antibodies contribute to understanding calcium-dependent pathways and developing therapeutic strategies targeting Ca²⁺-related pathologies.
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