| WB | 1/500-1/1000 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA |
| Entrez GeneID | 5585 |
| WB Predicted band size | Calculated MW: 104 kDa; Observed MW: 120 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | A synthetic peptide of human PKN |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于PKN1抗体的参考文献摘要，基于公开文献信息整理(部分为模拟示例，实际文献请通过学术数据库验证)：
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1. **文献名称**: *"PKN1 regulates epidermal growth factor-dependent β-catenin signaling"*
**作者**: Smith A, et al.
**摘要**: 研究通过特异性PKN1抗体发现，PKN1在EGFR信号通路中通过磷酸化β-catenin调控细胞迁移，抗体用于Western blot和免疫共沉淀实验验证相互作用。
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2. **文献名称**: *"Role of PKN1 in neuronal differentiation: Insights from antibody-mediated knockdown experiments"*
**作者**: Tanaka K, et al.
**摘要**: 利用PKN1抗体进行免疫荧光定位，发现PKN1在神经元轴突生长中富集，并通过抗体阻断实验证明其参与神经突触形成。
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3. **文献名称**: *"Development of a monoclonal antibody against human PKN1 for cancer biomarker studies"*
**作者**: Lee S, et al.
**摘要**: 报道了一种新型抗人PKN1单克隆抗体的开发，验证其在高通量ELISA和肿瘤组织芯片中的特异性，提示PKN1可能作为某些癌症的预后标志物。
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**注意**：以上内容为示例，真实文献需通过PubMed/Google Scholar检索关键词“PKN1 antibody”或“Protein kinase N1 antibody”获取。建议结合具体研究场景筛选文献。
**Background of PKN1 Antibody**
PKN1 (Protein Kinase N1), also known as PRK1 or PAK1. is a serine/threonine protein kinase belonging to the AGC kinase family. It plays a critical role in regulating diverse cellular processes, including cytoskeletal reorganization, cell migration, apoptosis, and membrane trafficking. PKN1 is activated by Rho GTPases (e.g., RhoA) via its N-terminal regulatory domain, which contains three antiparallel coiled-coil motifs. Its C-terminal catalytic domain shares homology with protein kinase C (PKC). PKN1 interacts with various signaling molecules, such as α-actinin and ARAP3. implicating it in pathways linked to cancer, neurodegenerative diseases, and immune responses.
PKN1-specific antibodies are essential tools for studying its expression, localization, and function. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to detect PKN1 in cell lines, tissues, or biological fluids. Research has highlighted PKN1's dual roles as both a tumor promoter and suppressor, depending on context, making it a potential therapeutic target. Antibodies targeting distinct epitopes (e.g., N-terminal vs. C-terminal regions) help dissect PKN1's regulatory mechanisms or post-translational modifications, such as phosphorylation.
Challenges in PKN1 antibody development include ensuring specificity due to homology with PKN2/PKN3 and avoiding cross-reactivity. Validated antibodies are crucial for elucidating PKN1's involvement in diseases and advancing biomarker discovery or targeted therapies.
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