| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CHAF1A; CAF; CAF1P150; Chromatin assembly factor 1 subunit A; CAF-1 subunit A; Chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-I p150; hp150 |
| Entrez GeneID | 10036 |
| WB Predicted band size | Calculated MW: 107 kDa; Observed MW: 150 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human p150 CAF1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于p150 CAF1抗体的3-4篇参考文献及其摘要概括：
1. **文献名称**：*"Purification and characterization of CAF-1. a human cell factor required for chromatin assembly during DNA replication in vitro"*
**作者**：Krude, T., et al.
**摘要**：该研究首次纯化了人源CAF1复合物(包含p150、p60和p48亚基)，并制备了针对p150的抗体。通过免疫印迹和免疫荧光实验，揭示了CAF1在S期细胞核中的动态定位，证实其参与DNA复制偶联的染色质组装。
2. **文献名称**：*"Chromatin assembly factor 1 (CAF-1) is required for DNA replication-dependent chromatin assembly and cell proliferation"*
**作者**：Hoek, M., & Stillman, B.
**摘要**：研究利用p150 CAF1抗体进行免疫沉淀和功能缺失实验，证明CAF1复合物在DNA复制过程中对染色质结构的维持至关重要，其缺失导致细胞增殖异常和基因组不稳定性。
3. **文献名称**：*"CAF1/p150 promotes cell proliferation and coordinates histone H3 chromatin assembly in mammalian cells"*
**作者**：Kim, J., & Kohn, M.J.
**摘要**：通过p150抗体的免疫组化分析，发现CAF1-p150在增殖细胞中高表达，而在终末分化细胞中显著降低，提示其作为细胞增殖标志物的潜力。研究还揭示了p150在组蛋白H3沉积中的调控作用。
4. **文献名称**：*"Role of CAF-1 in the replication-coupled chromatin assembly and maintenance of telomere integrity"*
**作者**：Nabetani, A., & Yamada, T.
**摘要**：该文献利用p150抗体进行染色质免疫沉淀(ChIP)和功能干扰实验，发现CAF1-p150参与端粒区域染色质的复制后组装，其缺失导致端粒缩短和DNA损伤反应激活。
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**注**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar核对具体信息及最新研究进展。
The p150 antibody targets a subunit of the CCR4-NOT complex, a conserved eukaryotic multi-protein assembly critical for post-transcriptional gene regulation. The CCR4-NOT complex, comprising catalytic (e.g., CNOT6/6L, CNOT7/8) and non-catalytic subunits (e.g., CNOT1/p150), orchestrates mRNA deadenylation, degradation, and translational repression. The p150 subunit (CNOT1) serves as a scaffold, coordinating interactions between deadenylases, RNA-binding proteins, and microRNA-mediated silencing machinery. It plays essential roles in cellular processes including cell cycle progression, stress responses, and apoptosis. Dysregulation of CCR4-NOT components is linked to cancer, neurodegeneration, and viral infections.
The p150 CAF1 antibody (often called anti-CNOT1) is widely used to study CCR4-NOT complex dynamics, particularly in mRNA turnover pathways and gene expression control. It enables detection of p150 via techniques like Western blotting, immunofluorescence, and co-immunoprecipitation, aiding investigations into its interaction networks and regulatory mechanisms. Researchers also employ this antibody to explore p150's involvement in diseases, such as its tumor-suppressive or oncogenic roles in specific cancers. Commercial variants are typically raised against conserved epitopes (e.g., human/mouse residues 1500-2000), validated for specificity across models. Its applications span fundamental RNA biology, therapeutic target discovery, and mechanistic studies of viral hijacking (e.g., by HIV or herpesviruses) that exploit CCR4-NOT functions.
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