| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H2A.2 |
| Entrez GeneID | 852283 |
| WB Predicted band size | Calculated MW: 14 kDa; Observed MW: 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of Histone H2A |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Histone H2A抗体的3篇参考文献示例(注：文献为虚构示例，仅作格式参考)：
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1. **文献名称**：*"A monoclonal antibody specific for histone H2A reveals dynamic chromatin remodeling during cell division"*
**作者**：Zhang Y, et al.
**摘要**：该研究开发了一种高特异性的H2A单克隆抗体，通过免疫印迹和免疫荧光验证其在多种物种中的保守性。研究发现H2A在细胞分裂过程中染色质结构重组的关键作用，抗体被成功用于活细胞成像和染色质免疫沉淀(ChIP)分析。
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2. **文献名称**：*"Histone H2A deubiquitination in DNA repair: antibody-based detection and functional implications"*
**作者**：Müller S, et al.
**摘要**：本文利用H2A抗体结合泛素化修饰检测技术，揭示了H2A去泛素化酶在DNA损伤修复中的机制。通过免疫共沉淀(Co-IP)和质谱分析，发现H2A的泛素化状态直接影响修复蛋白的招募效率。
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3. **文献名称**：*"Tissue-specific expression patterns of histone H2A variants revealed by a novel polyclonal antibody panel"*
**作者**：Ito T, et al.
**摘要**：研究团队制备了针对H2A不同变体(如H2A.X、H2A.Z)的多克隆抗体，并通过免疫组化系统比较了它们在哺乳动物组织中的分布差异。抗体被用于验证H2A变体在癌症模型中的异常表达，提示其作为潜在生物标志物的可能性。
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**注**：以上文献为模拟示例，实际研究中建议通过PubMed或Web of Science检索关键词(如“Histone H2A antibody applications”)获取真实文献。
Histone H2A is a core component of nucleosomes, playing a critical role in chromatin structure and gene regulation. As one of the five major histone families (H2A, H2B, H3. H4. and H1), H2A forms a heterodimer with H2B, stabilizing DNA wrapping around the nucleosome. Beyond structural functions, H2A undergoes post-translational modifications (e.g., ubiquitination, phosphorylation) that influence chromatin remodeling, DNA repair, and transcriptional control. Variants like H2A.X (involved in DNA damage response) and macroH2A (linked to gene silencing) further diversify its roles.
Antibodies targeting Histone H2A are essential tools for studying epigenetic mechanisms. They enable detection of specific isoforms, modification states, or nucleosome dynamics in techniques such as chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence. These antibodies help investigate processes like apoptosis-associated histone H2A phosphorylation (γH2A.X) or Polycomb-mediated gene silencing via H2A ubiquitination. Researchers also use them to explore disease mechanisms, including cancer (aberrant chromatin states) and neurodegenerative disorders (altered DNA repair pathways). Specificity validation (e.g., knockout controls) is crucial due to high homology among histone family members. Commercial H2A antibodies are typically developed using recombinant proteins or synthetic peptides corresponding to conserved or variant-specific epitopes.
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