| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HDAC3; Histone deacetylase 3; HD3; RPD3-2; SMAP45 |
| Entrez GeneID | 8841 |
| WB Predicted band size | Calculated MW: 49 kDa; Observed MW: 49 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human HDAC3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于HDAC3抗体的3篇参考文献，包括文献名称、作者和摘要概括：
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1. **文献名称**: *Class IIa HDACs regulate muscle integrity and longevity via autophagy in C. elegans*
**作者**: Li Y. et al.
**摘要**: 该研究通过免疫沉淀和Western blot技术，利用HDAC3特异性抗体探究了HDAC3在线虫肌肉稳态和自噬中的作用，发现其通过调控自噬相关基因表达延缓衰老。
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2. **文献名称**: *Cooperation between HDAC3 and DAX1 mediates repression of the anti-Müllerian hormone gene*
**作者**: Zhou L. et al.
**摘要**: 研究采用HDAC3抗体进行染色质免疫共沉淀(ChIP)，揭示了HDAC3与DAX1转录因子协同抑制抗缪勒管激素(AMH)表达的分子机制，及其在性腺发育中的功能。
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3. **文献名称**: *Antibody-based validation of HDAC3-specific inhibitors for therapeutic targeting*
**作者**: Bradner J.E. et al.
**摘要**: 通过对比多种HDAC3抗体的特异性，该研究验证了HDAC3抑制剂的选择性，并利用抗体检测了抑制后组蛋白乙酰化水平变化，为靶向HDAC3的癌症治疗提供依据。
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这些文献涵盖了HDAC3抗体在功能研究、分子机制解析及药物开发中的应用。如需更多文献或具体细节，可进一步说明。
Histone deacetylase 3 (HDAC3) is a class I enzyme within the HDAC family, which plays a critical role in epigenetic regulation by removing acetyl groups from lysine residues on histones and non-histone proteins. This deacetylation activity promotes chromatin condensation, leading to transcriptional repression. HDAC3 is essential for numerous biological processes, including cell proliferation, differentiation, metabolism, and apoptosis. It functions within multiprotein complexes, such as the nuclear receptor corepressor (N-CoR) and silencing mediator for retinoid and thyroid hormone receptors (SMRT) complexes, which target specific genomic regions to modulate gene expression. Dysregulation of HDAC3 has been implicated in cancers, neurodegenerative disorders, and metabolic diseases, making it a key therapeutic target.
HDAC3 antibodies are vital tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP). High-quality HDAC3 antibodies are validated for specificity, often through knockout (KO) cell lines or siRNA knockdown, to ensure minimal cross-reactivity with other HDAC isoforms (e.g., HDAC1. HDAC2). Researchers rely on these antibodies to explore HDAC3's role in disease mechanisms and assess the efficacy of HDAC inhibitors in preclinical models. Recent studies also highlight its context-dependent functions in immune regulation and circadian rhythm, expanding its relevance in both basic and translational research.
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