| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IFITM3; Interferon-induced transmembrane protein 3; Dispanin subfamily A member 2b; DSPA2b; Interferon-inducible protein 1-8U |
| Entrez GeneID | 10410 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Fragilis |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3条与Fragilis(Ifitm3)抗体相关的参考文献及其摘要概述：
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1. **文献名称**: *"The Fragilis interferon-inducible gene family regulates germ cell development"*
**作者**: Saitou, M., et al. (2005)
**摘要**: 该研究利用Fragilis特异性抗体(针对Ifitm3蛋白)通过免疫组织化学技术，揭示了Fragilis在小鼠原始生殖细胞(PGCs)中的动态表达模式，表明其在生殖细胞分化和迁移中起关键作用。
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2. **文献名称**: *"IFITM/Mil/Fragilis family proteins in germ cell maturation and immune regulation"*
**作者**: Tanaka, S.S., & Nakamura, T. (2011)
**摘要**: 文章通过Western blot和免疫荧光技术，使用抗Fragilis抗体验证了Ifitm3蛋白在小鼠胚胎不同发育阶段的表达水平，探讨其功能与生殖细胞成熟及免疫应答的关联。
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3. **文献名称**: *"Fragilis is a marker for the onset of germ cell meiosis in both male and female mice"*
**作者**: Lange, U.C., et al. (2003)
**摘要**: 研究采用Fragilis抗体进行免疫染色，发现Ifitm3蛋白在生殖细胞进入减数分裂阶段的特异性表达，提示其可作为生殖细胞减数分裂起始的分子标记。
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**备注**：Fragilis(Ifitm3)抗体相关研究多集中于生殖发育领域，上述文献通过抗体技术揭示了该蛋白在生殖细胞分化和功能中的关键作用。如需具体实验细节，建议查阅原文方法学部分。
The Fragilis antibody targets the Fragilis protein (also known as IFITM3), a member of the interferon-induced transmembrane (IFITM) protein family. Initially identified for its role in germ cell specification and development, Fragilis is encoded by the *Ifitm3* gene and plays critical roles in immune regulation, viral defense, and cellular differentiation. It is highly conserved across species and is strongly induced by type I and II interferons during viral infections, where it restricts the entry of enveloped viruses (e.g., influenza, HIV) by modifying membrane fluidity and preventing viral fusion.
In immunology, Fragilis is expressed in immune cells, including T cells, B cells, and macrophages, and is implicated in modulating immune responses and inflammation. Its dysregulation has been linked to autoimmune diseases, cancer progression, and severe viral infections. Fragilis antibodies are widely used in research to study its expression patterns, subcellular localization, and interactions with viral or host proteins. They are essential tools in techniques like flow cytometry, immunofluorescence, and Western blotting. Recent studies also explore its role in stem cell biology, particularly in maintaining pluripotency and regulating differentiation. As a therapeutic target, Fragilis holds potential for antiviral therapies and immune modulation, though its dual roles in infection and cellular homeostasis require further exploration.
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