| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FPGS; Tetrahydrofolate synthase |
| Entrez GeneID | 2356 |
| WB Predicted band size | Calculated MW: 65 kDa; Observed MW: 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human FPGS |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于FPGS抗体的3篇参考文献及其摘要内容：
1. **文献名称**: *"Characterization of Folylpolyglutamate Synthetase (FPGS) Monoclonal Antibody for Detection of Chemotherapy Resistance in Leukemia"*
**作者**: Smith J, et al.
**摘要**: 本研究开发了一种特异性识别FPGS的单克隆抗体，用于评估急性淋巴细胞白血病患者中FPGS的表达水平。研究显示，FPGS蛋白低表达与甲氨蝶呤耐药性显著相关，为临床化疗方案选择提供了潜在标志物。
2. **文献名称**: *"Subcellular Localization of FPGS in Hepatocytes: Insights from Immunofluorescence Staining"*
**作者**: Tanaka M, et al.
**摘要**: 通过高分辨率免疫荧光技术结合FPGS抗体，揭示了FPGS在肝细胞线粒体和胞质中的双重定位，表明其在叶酸代谢中的多区域功能，为研究叶酸缺乏相关疾病提供了新视角。
3. **文献名称**: *"Validation of a Novel FPGS Antibody for Western Blot Quantification in Colorectal Cancer Models"*
**作者**: Garcia R, et al.
**摘要**: 该研究验证了一种新型兔源多克隆FPGS抗体在蛋白质印迹中的灵敏度和特异性，证实其可用于定量结直肠癌细胞系中FPGS表达，并发现奥沙利铂耐药株中FPGS水平显著下调。
(注：上述文献为示例性内容，实际引用时需以真实文献为准。)
**Background of FPGS Antibodies**
Folypolyglutamate synthetase (FPGS) is a key enzyme in folate metabolism, catalyzing the polyglutamylation of folates and antifolate drugs. This post-translational modification enhances cellular retention of folates and increases the efficacy of antifolates by promoting their intracellular accumulation. FPGS plays a critical role in nucleotide synthesis, DNA repair, and one-carbon metabolism, making it essential for cell proliferation and survival.
FPGS antibodies are immunological tools developed to detect and quantify FPGS protein expression in research and clinical settings. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to study FPGS localization, expression levels, and regulation in various tissues and disease models. Dysregulation of FPGS has been linked to chemotherapy resistance, particularly in cancers treated with antifolate drugs like methotrexate, where reduced FPGS activity diminishes drug retention and efficacy.
Research using FPGS antibodies has also explored its role in neurodevelopmental disorders and age-related diseases, as folate metabolism is vital for neurological function and genomic stability. Additionally, FPGS antibodies aid in investigating species-specific differences, given the enzyme's high conservation across mammals. Recent studies focus on FPGS as a potential biomarker for predicting treatment response or as a target for modulating folate metabolism in cancer and other pathologies. These antibodies remain crucial for advancing understanding of folate biology and therapeutic strategies.
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