| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARA; IGDA; IHG1; FKHL7; IRID1; RIEG3; FREAC3; FREAC-3 |
| Entrez GeneID | 2296 |
| WB Predicted band size | Calculated MW: 57 kDa; Observed MW: 75 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | Recombinant protein of human FOXC1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于FOXC1抗体的3篇参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库查询验证)：
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1. **文献名称**: *FOXC1 regulates osteogenic potential of mesenchymal stem cells through the transactivation of membrane-type 1 matrix metalloproteinase*
**作者**: Berry FB, et al.
**摘要**: 该研究利用FOXC1抗体通过免疫沉淀和Western blot技术，揭示了FOXC1在间充质干细胞成骨分化中的作用，发现其通过调控基质金属蛋白酶(MT1-MMP)影响细胞外基质重塑。
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2. **文献名称**: *FOXC1 is a critical regulator of the cell cycle in basal-like breast cancer*
**作者**: Wang J, et al.
**摘要**: 研究通过免疫组化(使用FOXC1抗体)分析基底样乳腺癌组织，证实FOXC1过表达与细胞周期蛋白D1的调控相关，促进肿瘤增殖和不良预后。
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3. **文献名称**: *Mutations in FOXC1 contribute to Axenfeld-Rieger syndrome via dysregulation of extracellular matrix*
**作者**: Seifi M, et al.
**摘要**: 该研究使用FOXC1抗体进行小鼠模型免疫荧光染色，发现FOXC1基因突变导致角膜和虹膜发育异常，其机制与细胞外基质蛋白表达失调有关，为Axenfeld-Rieger综合征提供了分子机制解释。
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**建议验证平台**：可通过PubMed、Google Scholar搜索上述关键词，或查阅《Journal of Biological Chemistry》《Oncogene》等期刊近年文献获取详细信息。
FOXC1. a member of the Forkhead box (FOX) transcription factor family, plays critical roles in embryonic development, particularly in the formation of ocular structures, heart valves, and kidneys. It regulates gene expression by binding to DNA and is involved in maintaining mesenchymal cell phenotypes, extracellular matrix remodeling, and signaling pathways such as TGF-β and BMP. Mutations in FOXC1 are linked to developmental disorders like Axenfeld-Rieger syndrome, characterized by ocular and systemic abnormalities. Dysregulation of FOXC1 has also been implicated in cancers, including breast cancer and hepatocellular carcinoma, where it may promote metastasis and chemoresistance.
FOXC1 antibodies are essential tools for detecting protein expression and localization in research. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study FOXC1's roles in normal physiology and disease. Commercially available FOXC1 antibodies are typically raised against specific epitopes within the conserved Forkhead domain or variable regions. However, variability in antibody specificity (due to cross-reactivity with homologous FOX proteins like FOXC2) necessitates careful validation using knockout controls or siRNA-based approaches. Researchers must optimize protocols to account for tissue-specific expression levels and post-translational modifications. Reliable FOXC1 antibodies aid in exploring its regulatory networks, biomarker potential, and therapeutic targeting in pathological conditions.
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