| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FOXA1; Forkhead box A1; Forkhead box protein A1; HNF-3-alpha; TCF3A; HNF3A; Transcription factor 3A |
| Entrez GeneID | 3169 |
| WB Predicted band size | Calculated MW: 49 kDa; Observed MW: 49 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Recombinant protein of human FOXA1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于FOXA1抗体的代表性文献(精简格式)：
1. **"FOXA1 is a key mediator of hormonal response in breast cancer"**
*Carroll JS, et al. (2005)*
摘要：通过染色质免疫沉淀(ChIP)结合FOXA1抗体，揭示了FOXA1在乳腺癌细胞中作为雌激素受体(ER)的先锋因子，调控激素响应基因的染色质可及性。
2. **"Characterization of FOXA1 antibody specificity in prostate cancer models"**
*Gao N, et al. (2013)*
摘要：系统验证了FOXA1抗体在前列腺癌细胞中的特异性(Western Blot单一条带，免疫组化定位细胞核)，并证明FOXA1通过调控雄激素受体(AR)信号通路驱动肿瘤进展。
3. **"FOXA1 antibody validation for chromatin immunoprecipitation sequencing"**
*Bernardo GM, et al. (2010)*
摘要：比较多种FOXA1抗体在ChIP-seq中的性能，筛选出适用于染色质结合位点检测的高特异性抗体，并应用于乳腺上皮细胞转录调控网络研究。
4. **"Human Protein Atlas validation report for FOXA1 antibody HPA051784"**
*Uhlen M, et al. (2015)*
摘要：来自蛋白质图谱计划的技术报告，验证了FOXA1抗体在正常/肿瘤组织免疫组化中的可靠性，显示其在不同器官中表达模式与RNA水平高度一致。
注：以上文献信息为模拟示例，实际引用请核对真实出版物。
FOXA1 (Forkhead Box A1) is a transcription factor belonging to the forkhead box (FOX) protein family, characterized by a conserved winged-helix DNA-binding domain. It plays a pivotal role in organ development, differentiation, and metabolic regulation, particularly in hormone-responsive tissues such as the prostate, breast, and liver. FOXA1 acts as a pioneer factor, facilitating chromatin remodeling to enable the binding of other transcription factors, including steroid hormone receptors like the estrogen receptor (ER) and androgen receptor (AR). This function links FOXA1 to hormone-driven cancers, where its dysregulation is frequently observed.
FOXA1 antibodies are essential tools for studying its expression, localization, and interaction partners in both normal and pathological contexts. In research, these antibodies are widely used in techniques like chromatin immunoprecipitation sequencing (ChIP-seq), immunohistochemistry (IHC), and Western blotting to explore FOXA1's role in gene regulation and cancer progression. Clinically, FOXA1 expression is assessed as a biomarker in breast and prostate cancers, where its levels correlate with tumor subtype, aggressiveness, and therapeutic response. For instance, FOXA1 is often co-expressed with ER in luminal breast cancers and is implicated in prostate cancer lineage plasticity. Antibodies specific to FOXA1 must be validated for cross-reactivity and epitope specificity due to structural similarities within the FOX family. Their reliability supports mechanistic studies and diagnostic applications, making them critical in both basic and translational research.
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