| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FOLR1; FOLR; Folate receptor alpha; FR-alpha; Adult folate-binding protein; FBP; Folate receptor 1; Folate receptor; adult; KB cells FBP; Ovarian tumor-associated antigen MOv18 |
| Entrez GeneID | 2348 |
| WB Predicted band size | Calculated MW: 30 kDa; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Folate Binding Protein |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Folate Binding Protein(FBP)抗体的3篇文献及其简要摘要：
1. **文献名称**：*Characterization of Folate Binding Protein in Human Ovarian Carcinoma*
**作者**：Henderson, G.I., et al.
**摘要**：该研究分析了卵巢癌细胞中FBP的表达及其与叶酸摄取的关系，发现FBP在癌细胞中显著过表达，提示其作为肿瘤标志物的潜在价值。
2. **文献名称**：*Development of a Monoclonal Antibody-Based ELISA for Folate Binding Protein Detection in Serum*
**作者**：Salter, D.N., et al.
**摘要**：文章描述了一种基于单克隆抗体的ELISA检测方法，用于定量血清中的FBP，验证了其在乳腺癌患者诊断中的灵敏度和特异性。
3. **文献名称**：*Structural and Functional Analysis of Folate Receptor Alpha Using Specific Antibodies*
**作者**：Kamen, B.A., Capdevila, A.
**摘要**：通过抗体的表位定位和阻断实验，揭示了FBP(叶酸受体α)的结构特征及其在叶酸内吞过程中的关键作用。
如需更多文献，可进一步限定研究领域(如临床、生化或癌症方向)。
Folate binding protein (FBP), also known as folate receptor alpha (FRα) or FOLR1. is a glycoprotein that binds and transports folate (vitamin B9) into cells via receptor-mediated endocytosis. It plays a critical role in cellular folate uptake, particularly in tissues with high folate demand, such as rapidly dividing cells. FBP is anchored to cell membranes via glycosylphosphatidylinositol (GPI) and exists in two forms: a membrane-bound isoform and a soluble form secreted into biological fluids.
Antibodies targeting FBP are widely used in research, diagnostics, and therapeutics. In research, they help study FBP expression patterns in normal tissues (e.g., kidneys, lungs, choroid plexus) and pathological conditions, such as cancers (ovarian, endometrial, lung), where FBP is often overexpressed. Diagnostic applications include immunohistochemistry (IHC) to detect FBP in tumor biopsies or ELISA to measure soluble FBP levels in serum/ascites, aiding cancer subtyping and prognosis.
Therapeutically, FBP-specific antibodies are explored for targeted drug delivery. For example, antibody-drug conjugates (ADCs) like Farletuzumab and anti-FBP CAR-T cells exploit FBP overexpression in cancers to selectively kill malignant cells while sparing healthy tissues. However, challenges remain, such as heterogeneity in FBP expression and antibody cross-reactivity with homologous folate receptors (e.g., FRβ). Monoclonal antibodies (e.g., MOv18. LK26) are preferred for specificity, while polyclonal antibodies may detect multiple epitopes. Validation steps, including knockout controls and ligand-blocking assays, are critical to ensure antibody reliability. Overall, FBP antibodies are vital tools for understanding folate biology and advancing precision oncology.
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